The ZFHX1B gene is a member of the delta-EF1/Zfh1 family of 2-handed zinc finger/homeodomain proteins. ZFHX1B is strongly transcribed at an early stage in the developing peripheral and central nervous systems of both mice and humans, in all neuronal regions of the brains of 25-week human fetuses and adult mice, and in numerous nonneural tissues.The SMADIP1 gene (also known as SIP1) is a member of the delta-EF1 (ZEB1; MIM 189909)/Zfh1 family of 2-handed zinc finger/homeodomain proteins. SMADIP1 interacts with receptor-mediated, activated full-length SMADs (see MIM 605568) (Verschueren et al., 1999 [PubMed 10400677]).1) Verstappen, G., (2008) Hum. Mol. Genet. 17 (8), 1175-1183.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ZEB2.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
ZEB2 antibody can be used for detection of ZEB2 by ELISA at 1:2500. ZEB2 antibody can be used for detection of ZEB2 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Human brain at 0.2-1 ug/ml.
Application
Antibody used in IHC on Mouse at 1:200.
Positive Control
1) Cat. No. 1211 - HepG2 Cell Lysate
Application Notes
ZEB2 antibody can be used for detection of ZEB2 by ELISA at 1:2500. ZEB2 antibody can be used for detection of ZEB2 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store ZEB2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.