Application
Western blot analysis of ZEB2 in EL4 cell lysate with ZEB2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.Application
Immunofluorescence of ZEB2 in Jurkat cells with ZEB2 antibody at 20 μg/ml.Application
Immunofluorescence of ZEB2 in Jurkat cells with ZEB2 antibody at 20 μg/mL.Application
Immunocytochemistry of ZEB2 in Jurkat cells with ZEB2 antibody at 20 μg/mL.| Product Name | ZEB2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | ZEB2 Antibody: ZEB2, initially identified as Smad interacting-protein 1, is normally located in the nucleus and functions as a DNA-binding transcriptional repressor that interacts with activated SMADs. Like the homologous ZEB1, ZEB2 inhibits the transcription of the E-cadherin gene and induces epithelial-mesenchymal transition, a genetic program controlling cell migration during embryonic development and wound healing, in vitro. ZEB2 can also protect cells from DNA damage-induced apoptosis, suggesting that its expression may contribute to tumor progression. Recent evidence has shown that ZEB2 is often observed in the cytoplasm in numerous cancer tissues, indicating that its localization may be regulated in normal and tumor tissues. Mutations in this gene are also associated with Hirschsprung disease/Mowat-Wilson syndrome.1) Comjin J, Berx G, Vermassen P, et al. The two-handed E box binding zinc finger protein SIP1 down regulates E-cadherin and induces invasion. Mol. Cell2001; 7:1267-78. |
| Immunogen | ZEB2 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human ZEB2. The immunogen is located within the last 50 amino acids of ZEB2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| ZEB2 antibody can be used for detection of ZEB2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ZEB2 |
|---|---|
| Alternative Names | ZEB2 Antibody: SIP1 SIP-1 ZFHX1B HSPC082 SMADIP1 KIAA0569 SIP1 ZFX1B HRIHFB2411 Zinc finger E-box-binding homeobox 2 Smad-interacting protein 1 |
| Molecular Weight(MW) | Predicted: 134 kDa Observed: 135 kDa |
| Sequence Similarities | 2) Cat. No. 17-005 - Jurkat Cell Slide |
| Tissue Specificity | ZEB2 antibody is predicted to not cross-react with ZEB1. |
Application
Western blot analysis of ZEB2 in EL4 cell lysate with ZEB2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Application
Immunofluorescence of ZEB2 in Jurkat cells with ZEB2 antibody at 20 μg/ml.
Application
Immunofluorescence of ZEB2 in Jurkat cells with ZEB2 antibody at 20 μg/mL.
Application
Immunocytochemistry of ZEB2 in Jurkat cells with ZEB2 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1287 - EL4 Cell Lysate |
|---|---|
| Application Notes | ZEB2 antibody can be used for detection of ZEB2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | ZEB2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ZEB2 Antibody is supplied in PBS containing 0.02% sodium azide. |
