Application
Western blot analysis of ZFP219 in EL4 cell lysate with ZFP219 antibody at 1 μg/mL.Application
Immunofluorescence of ZFP219 in mouse brain tissue with ZFP219 antibody at 20 μg/mL.Application
Immunohistochemistry of ZFP219 in mouse brain tissue with ZFP219 antibody at 5 μg/mL.| Product Name | ZFP219 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ZFP219 Antibody: ZFP219, Zinc finger protein 219 |
| Product description | ZFP219 Antibody: ZFP219 is a developmentally regulated member of the Kruppel-like zinc finger gene family that is thought to function as a transcriptional repressor. Yeast two-hybrid screening showed association with Sox9, a transcription factor that is essential for chondrogenesis. ZFP219 is specifically expressed in the developing limb buds and colocalizes with Sox9 in the nucleus. Knockdown of ZFP219 expression decreased Sox9-induced mRNA expression, and a dominant-negative mutant of ZFP219 inhibited Bmp2-induced chondrocyte differentiation, suggesting that ZFP219 plays an important role as a transcriptional partner of Sox9 in the regulation of chondrocyte differentiation.1) Sakai T, Toyoda A, Hashimoto K, et al. Isolation and characterization of a novel zinc finger gene, ZNF219, and mapping to the human chromosome 14q11 region. DNA Res. 2000; 7:137-41. |
| Immunogen | ZFP219 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human ZFP219. The immunogen is located within amino acids 90 - 140 of ZFP219. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| ZFP219 antibody can be used for detection of ZFP219 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ZNF219 |
|---|---|
| Alternative Names | ZFP219 Antibody: ZFP219 Zinc finger protein 219 |
| Molecular Weight(MW) | Predicted: 80 kDa Observed: 78 kDa |
| Tissue Specificity | At least two isoforms of ZFP219 are known to exist; this antibody will recognize both. |
| Entrez Gene | 51222 |
|---|---|
| Protein Accession | NP_001095142 |
Application
Western blot analysis of ZFP219 in EL4 cell lysate with ZFP219 antibody at 1 μg/mL.
Application
Immunofluorescence of ZFP219 in mouse brain tissue with ZFP219 antibody at 20 μg/mL.
Application
Immunohistochemistry of ZFP219 in mouse brain tissue with ZFP219 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1287 - EL4 Cell Lysate |
|---|---|
| Application Notes | ZFP219 antibody can be used for detection of ZFP219 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ZFP219 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ZFP219 Antibody is supplied in PBS containing 0.02% sodium azide. |
