Application
Western blot analysis of ZFP281 in A-20 cell lysate with ZFP281 antibody at 0.5 μg/mL.Application
Immunofluorescence of ZFP291 in A20 cells with ZFP291 antibody at 20 μg/mL.| Product Name | ZFP281 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ZFP281 Antibody: ZBP-99, ZNP-99, GZP1, ZBP99, Zinc finger protein 281, GC-box-binding zinc finger protein 1 |
| Product description | ZFP281 Antibody: ZFP281, also known as ZBP-99, is a member of a conserved family of transcription factors that interact with GC-rich promoters. It contains four Kruppel-like zinc fingers and is highly homologous to ZBP-89, another regulator of ornithine decarboxylase gene expression. ZFP281 also interacts with many other other transcription factors, such as Sp1 to repress vimentin gene transcription. ZFP281 has also been shown to control embryonic stem cell pluripotency by direct regulating specific target genes. ZFP281 phyically interacts with the transcription factos POU5F1, Sox2, and NANOG. Gene expression microarray anlysis indicated that some ZFP281 target genes were activated, while others were repressed upon knockdown of ZFP281 expression, showing that ZFP281 plays bifuctinal roles in regulating gene expression in stem cells.1) Law DJ, Du M, Law GL, et al. ZBP-99 defines a conserved family of transcription factors and regulates ornithine decarboxylase gene expression. Biochem. Biophys. Res. Commun. 1999; 262:113-20. |
| Immunogen | ZFP281 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human ZFP281. The immunogen is located within amino acids 600 - 650 of ZFP281. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| ZFP281 antibody can be used for detection of ZFP281 by Western blot at 0.5 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ZNF281 |
|---|---|
| Alternative Names | ZFP281 Antibody: ZBP-99 ZNP-99 GZP1 ZBP99 Zinc finger protein 281 GC-box-binding zinc finger protein 1 |
| Sequence Similarities | 2) Cat. No. 17-208 - A-20 Cell Slide |
Application
Western blot analysis of ZFP281 in A-20 cell lysate with ZFP281 antibody at 0.5 μg/mL.
Application
Immunofluorescence of ZFP291 in A20 cells with ZFP291 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1288 - A20 Cell Lysate |
|---|---|
| Application Notes | ZFP281 antibody can be used for detection of ZFP281 by Western blot at 0.5 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ZFP281 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ZFP281 Antibody is supplied in PBS containing 0.02% sodium azide. |
