Zfpm1 is a transcription regulator that plays an essential role in erythroid and megakaryocytic cell differentiation. It is essential cofactor that acts via the formation of a heterodimer with transcription factors of the GATA family. Zfpm1 may be involved in regulation of some genes in gonads and involved in cardiac development, in a non-redundant way with ZFPM2/FOG2.1) Jacobsen, C.M., et al., (2005) Dev. Dyn. 234 (2), 355-362.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse ZFPM1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
ZFPM1 antibody can be used for detection of ZFPM1 by ELISA at 1:62500. ZFPM1 antibody can be used for detection of ZFPM1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Mouse SP2/0 at 0.2-1 ug/ml.
Positive Control
1) SP2/0 Cell Lysate
Application Notes
ZFPM1 antibody can be used for detection of ZFPM1 by ELISA at 1:62500. ZFPM1 antibody can be used for detection of ZFPM1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store ZFPM1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.