Application
Western blot analysis of ZFYVE16 Antibody in K562 cell line lysates (35ug/lane)Application
ZFYVE16 Antibody IHC analysis in formalin fixed and paraffin embedded lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ZFYVE16 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The ZFYVE16 gene encodes endofin, an endosomal protein implicated in regulating membrane trafficking. It is characterized by the presence of a phosphatidylinositol 3-phosphate-binding FYVE domain positioned in the middle of the molecule.1) Toy, W., et al. Cell. Signal. 22(3):437-446(2010) Chen, Y., et al. Proteomics 7(14):2384-2397(2007) Shi, W., et al. J. Cell. Sci. 120 (PT 7), 1216-1224 (2007) |
| Immunogen | This ZFYVE16 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 375-404 amino acids from the N-terminal region of human ZFYVE16. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ZFYVE16 |
|---|---|
| Alternative Names | Zinc finger FYVE domain-containing protein 16 Endofin Endosome-associated FYVE domain protein ZFYVE16 KIAA0305 |
| Molecular Weight(MW) | 169 kDa |
Application
Western blot analysis of ZFYVE16 Antibody in K562 cell line lysates (35ug/lane)
Application
ZFYVE16 Antibody IHC analysis in formalin fixed and paraffin embedded lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
