Application
Western blot analysis of ZIP10 in human spleen tissue lysate with ZIP10 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunohistochemistry of ZIP10 in human spleen tissue with ZIP10 antibody at 2.5 μg/mL.| Product Name | ZIP10 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | ZIP10 Antibody: ZIP10, also known as Slc39A10, is a widely expressed zinc transporter with nine transmembrane domains. Zinc is an essential ion for cells and plays significant roles in the growth, development, and differentiation. ZIP10 mRNA was found to be significantly decreased in the intestines and kidneys of hypothyroid rats and increased in those of hyperthyroid rats, indicating that ZIP10 is positively regulated by thyroid hormones. ZIP10 mRNA was also found to be upregulated in invasive and metastatic breast cancer and cell lines, suggesting that ZIP10 could serve as a possible marker for the metastatic phenotype and possibly a target for novel treatment strategies. At least three isoforms of ZIP10 are known to exist.1) Kaler P and Prasad R. Molecular cloning and functional characterization of novel transporter rZip10 (Slc39a10) involved in zinc uptake across renal brush-border membrane. Am. J. Renal Physiol.2007; 292:F217-29. |
| Immunogen | ZIP10 antibody was raised against a 17 amino acid synthetic peptide near the center of the human ZIP10. The immunogen is located within amino acids 490 - 540 of ZIP10. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| ZIP10 antibody can be used for detection of ZIP10 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Slc39a10 |
|---|---|
| Alternative Names | ZIP10 Antibody: |
| Sequence Similarities | 2) Cat. No. 10-901 - Human Spleen Tissue Slide |
| Tissue Specificity | This antibody will not cross-react with the zinc transporter ZIP11. |
Application
Western blot analysis of ZIP10 in human spleen tissue lysate with ZIP10 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunohistochemistry of ZIP10 in human spleen tissue with ZIP10 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1306 - Human Spleen Tissue Lysate |
|---|---|
| Application Notes | ZIP10 antibody can be used for detection of ZIP10 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | ZIP10 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ZIP10 Antibody is supplied in PBS containing 0.02% sodium azide. |
