Application
Western blot analysis of ZIP12 in HepG2 cell lysate with ZIP12 antibody at (A) 0.5 and (B) 1 μg/mL.Application
Immunofluorescence of ZIP12 in mouse liver tissue with ZIP12 antibody at 20 μg/mL.Application
Immunohistochemistry of ZIP12 in mouse liver tissue with ZIP12 antibody at 2.5 μg/mL.| Product Name | ZIP12 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | ZIP12 Antibody: The zinc transporter ZIP12, also known as SLC39A12, is a member of a family of divalent ion transporters. Zinc is an essential ion for cells and plays significant roles in the growth, development, and differentiation. The zinc transporter family is divided into four subfamilies (I, II, LIV-1 and gufA). ZIP12 is a multipass membrane protein that belongs to the ZIP transporter subfamily LIV-1. Increased levels of ZIP12 have been observed during acute inflammation in the airway and asthma, suggesting that it may be promote increased zinc uptake, as this can reduce inflammation. The zinc content in brains in individuals with schizophrenia is lower than in individuals with other cerebral diseases, and mutations in ZIP12 have been observed in a small group of schizophrenic patients, suggesting that ZIP12 may play a role in this disease.1) Dufner-Beattie J, Langmade SJ, Wang F, et al. Structure, function, and regulation of a subfamily of mouse zinc transporter genes. J. Biol. Chem.2003; 278:50142-50. |
| Immunogen | ZIP12 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human ZIP12. The immunogen is located within the first 50 amino acids of ZIP12. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| ZIP12 antibody can be used for detection of ZIP12 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Slc39a12 |
|---|---|
| Alternative Names | ZIP12 Antibody: ZIP-12 LZT-Hs8 bA570F3.1 ZIP12 Zinc transporter ZIP12 LIV-1 subfamily of ZIP zinc transporter 8 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (77%) |
| Tissue Specificity | At least four isoforms of ZIP12 are known to exist. ZIP12 antibody is predicted to not cross-react with other ZIP family members. |
| Entrez Gene | 221074 |
|---|---|
| Protein Accession | NP_001138667 |
Application
Western blot analysis of ZIP12 in HepG2 cell lysate with ZIP12 antibody at (A) 0.5 and (B) 1 μg/mL.
Application
Immunofluorescence of ZIP12 in mouse liver tissue with ZIP12 antibody at 20 μg/mL.
Application
Immunohistochemistry of ZIP12 in mouse liver tissue with ZIP12 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1306 - Human Spleen Tissue Lysate |
|---|---|
| Application Notes | ZIP12 antibody can be used for detection of ZIP12 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | ZIP12 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ZIP12 Antibody is supplied in PBS containing 0.02% sodium azide. |
