Application
Western blot analysis of ZIP13 in K562 cell lysate with ZIP13 antibody at 1 μg/mL.Application
Immunofluorescence of ZIP13 in human spleen tissue with ZIP13 antibody at 20 μg/mL.Application
Immunohistochemistry of ZIP13 in human spleen tissue with ZIP13 antibody at 2.5 μg/mL.| Product Name | ZIP13 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | ZIP13 Antibody: The zinc transporter ZIP13, also known as SLC39A13, is a member of a family of divalent ion transporters. Zinc is an essential ion for cells and plays significant roles in the growth, development, and differentiation. The zinc transporter family is divided into four subfamilies (I, II, LIV-1 and gufA). ZIP13 is a multipass membrane protein that belongs to the ZIP transporter subfamily LIV-1. Mutations in ZIP13 have recently been shown to cause a spondylocheiro dysplastic form of Ehlers-Danlos syndrome (SCD-EDS), a generalized skeletal dysplasia involving mainly the spine with clinical abnormalities of the hands in addition to EDS-like features. Other experiments have shown that ZIP13 is required for proper connective tissue development and is involved in BMP/TGF- signaling pathways.1) Dufner-Beattie J, Langmade SJ, Wang F, et al. Structure, function, and regulation of a subfamily of mouse zinc transporter genes. J. Biol. Chem.2003; 278:50142-50. |
| Immunogen | ZIP13 antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human ZIP13. The immunogen is located within amino acids 150 - 200 of ZIP13. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| ZIP13 antibody can be used for detection of ZIP13 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Slc39a13 |
|---|---|
| Alternative Names | ZIP13 Antibody: ZIP13 Zinc transporter ZIP13 LIV-1 subfamily of ZIP zinc transporter 9 LZT-Hs9 |
| Sequence Similarities | 2) Cat. No. 10-901 - Human Spleen Tissue Slide |
| Tissue Specificity | At least two isoforms of ZIP13 are known to exist; this antibody will detect both isoforms. ZIP13 antibody is predicted to not cross-react with other ZIP family members. |
| Entrez Gene | 91252 |
|---|---|
| Protein Accession | NP_001121697 |
Application
Western blot analysis of ZIP13 in K562 cell lysate with ZIP13 antibody at 1 μg/mL.
Application
Immunofluorescence of ZIP13 in human spleen tissue with ZIP13 antibody at 20 μg/mL.
Application
Immunohistochemistry of ZIP13 in human spleen tissue with ZIP13 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | ZIP13 antibody can be used for detection of ZIP13 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | ZIP13 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ZIP13 Antibody is supplied in PBS containing 0.02% sodium azide. |
