Application
Western blot analysis of ZIP2 in (A) mouse and (B) rat brain tissue lysate with ZIP2 antibody at 1 μg/mL.Application
Immunofluorescence of ZIP2 in human brain tissue with ZIP2 antibody at 20 μg/mL.Application
Immunohistochemistry of ZIP2 in human brain tissue with ZIP2 antibody at 2.5 μg/mL.| Product Name | ZIP2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | ZIP2 Antibody: The zinc transporter ZIP2, also known as SLC39A2, is a member of a family of divalent ion transporters. Zinc is an essential ion for cells and plays significant roles in the growth, development, and differentiation. Similar to knock-outs of ZIP1 and ZIP3, ZIP2-null mice have no phenotypic differences compared to wild-type mice. Only when ZIP1, ZIP2, and ZIP3 genes are all eliminated and these mutant mice are fed a zinc-deficient diet do abnormalities such as reduced embryonic-membrane bound alkaline phosphatase activity and abnormal development occur, indicating that the ZIP1-3 proteins play an important, noncompensatory role when zinc is deficient. More recent studies have shown that ZIP2 and ZIP3 are down regulated in human prostate adenocarcinomatous glands, and may be important in the retention of zinc in the cellular compartment.1) Gaither LA and Eide DJ. Functional expression of the human hZIP2 zinc transporter. J. Biol. Chem.2000; 275:5560-4. |
| Immunogen | ZIP2 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human ZIP2. The immunogen is located within amino acids 70 - 120 of ZIP2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| ZIP2 antibody can be used for detection of ZIP2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SLC39A2 |
|---|---|
| Alternative Names | ZIP2 Antibody: 6A1 ZIP2 ETI-1 ZIP-2 Zinc transporter ZIP2 6A1 |
| Molecular Weight(MW) | Predicted: 34kDa Observed: 32 kDa |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Western blot analysis of ZIP2 in (A) mouse and (B) rat brain tissue lysate with ZIP2 antibody at 1 μg/mL.
Application
Immunofluorescence of ZIP2 in human brain tissue with ZIP2 antibody at 20 μg/mL.
Application
Immunohistochemistry of ZIP2 in human brain tissue with ZIP2 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1403 - Mouse Brain Tissue Lysate |
|---|---|
| Application Notes | ZIP2 antibody can be used for detection of ZIP2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | ZIP2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ZIP2 Antibody is supplied in PBS containing 0.02% sodium azide. |
