Application
Western blot analysis of ZIP3 in mouse lung tissue lysate with ZIP3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.Application
Immunohistochemistry of ZIP3 in mouse lung tissue with ZIP3 antibody at 5 μg/mL.| Product Name | ZIP3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | ZIP3 Antibody: The zinc transporter ZIP3, also known as SLC39A3, is a member of a family of divalent ion transporters. Zinc is an essential ion for cells and plays significant roles in the growth, development, and differentiation. Similar to knock-outs of ZIP1 and ZIP2, ZIP3-null mice have no phenotypic differences compared to wild-type mice. Only when ZIP1, ZIP2, and ZIP3 genes are all eliminated and these mutant mice are fed a zinc-deficient diet do abnormalities such as reduced embryonic-membrane bound alkaline phosphatase activity and abnormal development occur, indicating that the ZIP1-3 proteins play an important, noncompensatory role when zinc is deficient. More recent studies have shown that ZIP2 and ZIP3 are down regulated in human prostate adenocarcinomatous glands, and may be important in the retention of zinc in the cellular compartment.1) Dufner-Beattie J, Langmade SJ, Wang F, et al. Structure, function, and regulation of a subfamily of mouse zinc transporter genes. J. Biol. Chem.2003; 278:50142-50. |
| Immunogen | ZIP3 antibody was raised against a 16 amino acid synthetic peptide near the center of human ZIP3. The immunogen is located within amino acids 110 - 160 of ZIP3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| ZIP3 antibody can be used for detection of ZIP3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SLC39A3 |
|---|---|
| Alternative Names | ZIP3 Antibody: ZIP3 ZIP-3 ZIP3 Zinc transporter ZIP3 Solute carrier family 39 member 3 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat: (94%), Bovine: (88%) |
Application
Western blot analysis of ZIP3 in mouse lung tissue lysate with ZIP3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Application
Immunohistochemistry of ZIP3 in mouse lung tissue with ZIP3 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1408 - Mouse Small Intestine Tissue Lysate |
|---|---|
| Application Notes | ZIP3 antibody can be used for detection of ZIP3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | ZIP3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ZIP3 Antibody is supplied in PBS containing 0.02% sodium azide. |
