Application
Western blot analysis of ZIP8 in human spleen tissue lysate with ZIP8 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of ZIP8 in human spleen tissue with ZIP8 antibody at 20 μg/mL.Application
Immunohistochemistry of ZIP8 in human spleen tissue with ZIP8 antibody at 5 μg/mL.| Product Name | ZIP8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | ZIP8 Antibody: The zinc transporter ZIP8, also known as SLC39A9, is a member of a family of divalent ion transporters. Zinc is an essential ion for cells and plays significant roles in the growth, development, and differentiation. The zinc transporter family is divided into four subfamilies (I, II, LIV-1 and gufA). ZIP8 is glycosylated and located at the plasma membrane and mitochondria. It has been identified as the transporter responsible for transport of the toxic Cadmium cation. ZIP8 has also been suggested to play a role in the regulation of interferon-gamma expression in activated human T cells.1) Dufner-Beattie J, Langmade SJ, Wang F, et al. Structure, function, and regulation of a subfamily of mouse zinc transporter genes. J. Biol. Chem.2003; 278:50142-50. |
| Immunogen | ZIP8 antibody was raised against a 18 amino acid synthetic peptide near the carboxy terminus of human ZIP8. The immunogen is located within the last 50 amino acids of ZIP8. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| ZIP8 antibody can be used for detection of ZIP8 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Slc39a8 |
|---|---|
| Alternative Names | ZIP8 Antibody: ZIP8 PP3105 BIGM103 LZT-Hs6 ZIP8 Zinc transporter ZIP8 BCG-induced integral membrane protein in monocyte clone 103 protein |
| Sequence Similarities | 2) Cat. No. 10-901 - Human Spleen Tissue Slide |
| Tissue Specificity | ZIP8 antibody is predicted to not cross-react with other ZIP family members. |
Application
Western blot analysis of ZIP8 in human spleen tissue lysate with ZIP8 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of ZIP8 in human spleen tissue with ZIP8 antibody at 20 μg/mL.
Application
Immunohistochemistry of ZIP8 in human spleen tissue with ZIP8 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1306 - Human Spleen Tissue Lysate |
|---|---|
| Application Notes | ZIP8 antibody can be used for detection of ZIP8 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | ZIP8 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ZIP8 Antibody is supplied in PBS containing 0.02% sodium azide. |
