Application
Western Blot at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: human kidney lysate Lysates/proteins at 20 ug per lane.Application
Antibody is used in Western blot to detect ZMPSTE24 in mouse cerebellum tissue lysate.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | ZMPSTE24 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | ZMPSTE24 is a zinc metalloprotease similar to yeast Ste24p. It is an integral membrane protein belonging to peptidase family M48 and is found in the endoplasmic reticulum and possibly in the Golgi compartment. It is thought to be involved in the proteolytic processing of farnesylated proteins.1) Freije, J.M., et al., Genomics 58(3):270-280 (1999). |
| Immunogen | This ZMPSTE24 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 400-430 amino acids from the Central region of human ZMPSTE24. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | ZMPSTE24 |
|---|---|
| Alternative Names | CAAX prenyl protease 1 homolog Farnesylated proteins-converting enzyme 1 FACE-1 Prenyl protein-specific endoprotease 1 Zinc metalloproteinase Ste24 homolog ZMPSTE24 FACE1 STE24 |
| Molecular Weight(MW) | 55 kDa |
Application
Western Blot at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: human kidney lysate Lysates/proteins at 20 ug per lane.
Application
Antibody is used in Western blot to detect ZMPSTE24 in mouse cerebellum tissue lysate.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
