Application
Western blot analysis of ZMYM2 in EL4 cell lysate with ZMYM2 antibody at (A) 0.125 and (B) 0.25 μg/mL.Application
Immunofluorescence of ZMYM2 in HeLa cells with ZMYM2 antibody at 20 μg/mL.Application
Immunocytochemistry of ZMYM2 in HeLa cells with ZMYM2 antibody at 10 μg/mL.| Product Name | ZMYM2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | ZMYM2 Antibody: Zinc-finger proteins contain DNA-binding domains characterized by the unique role of zinc and have a wide variety of functions such as transcriptional activation or repression. The protein folding and the DNA binding ability are governed by the coordination of a zinc ion. As a member of the MYM (myeloproliferative and mental retardation) gene family, ZMYM1 is widely expressed in different tissues in eukaryotes under several forms derived by alternative splicing. While its function remains unknown, the related protein ZMYM2 has been shown to associate with and stabilize the LSD1-CoREST-HDAC1 (LCH) complex of chromatin through its MYM-type zinc fingers, thereby enhancing the transcriptional repression of several genes, suggesting that ZMYM1 may play a similar role.1) Rosenfeld R and Margalit H. Zinc fingers: conserved properties that can distinguish between spurious and actual DNA-binding motifs. J. Biomol. Struct. Dyn.1993; 11:557-70. |
| Immunogen | ZMYM1 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human ZMYM1. The immunogen is located within amino acids 120 - 170 of ZMYM2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| ZMYM2 antibody can be used for detection of ZMYM2 by Western blot at 0. 125 - 0.25 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ZMYM2 |
|---|---|
| Alternative Names | ZMYM2 Antibody: FIM MYM RAMP SCLL ZNF198 FIM Zinc finger MYM-type protein 2 Fused in myeloproliferative disorders protein |
| Sequence Similarities | 2) Cat. No. 17-001 - HeLa Cell Slide |
| Tissue Specificity | At least two isoforms of ZMYM1 are known to exist; this antibody will detect only the shorter isoform. ZMYM1 antibody is predicted to not cross-react with other ZMYM protein family members. |
Application
Western blot analysis of ZMYM2 in EL4 cell lysate with ZMYM2 antibody at (A) 0.125 and (B) 0.25 μg/mL.
Application
Immunofluorescence of ZMYM2 in HeLa cells with ZMYM2 antibody at 20 μg/mL.
Application
Immunocytochemistry of ZMYM2 in HeLa cells with ZMYM2 antibody at 10 μg/mL.| Positive Control | 1) Cat. No. 1462 - Rat Lung Tissue Lysate |
|---|---|
| Application Notes | ZMYM2 antibody can be used for detection of ZMYM2 by Western blot at 0. 125 - 0.25 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | ZMYM2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ZMYM2 Antibody is supplied in PBS containing 0.02% sodium azide. |
