ZNF136 belongs to the krueppel C2H2-type zinc-finger protein family. It contains 14 C2H2-type zinc fingers and 1 KRAB domain. ZNF136 may be involved in transcriptional regulation as a weak repressor when alone, or a potent one when fused with a heterologous protein containing a KRAB B-domain.1) Vissing, H., FEBS Lett. 369 (2-3), 153-157 (1995).
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ZNF136.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
ZNF136 antibody can be used for detection of ZNF136 by ELISA at 1:12500. ZNF136 antibody can be used for detection of ZNF136 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human 721_B at 0.2-1 ug/ml.
Positive Control
1) 721_B Cell Lysate
Application Notes
ZNF136 antibody can be used for detection of ZNF136 by ELISA at 1:12500. ZNF136 antibody can be used for detection of ZNF136 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store ZNF136 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.