Application
Western blot analysis in K562 cell line lysates (35ug/lane).Application
Western blot analysis in mouse heart tissue lysates (35ug/lane).Application
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ZNF146 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Zinc finger protein OZF, Only zinc finger protein, Zinc finger protein 146, ZNF146, OZF |
| Product description | The ZNF146 protein is a 33 kDa Kruppel protein, composed solely of 10 zinc finger motifs. ZNF146 overexpression in tumours may alter the balance between hRap1 and other telomeric proteins; therefore ZNF146 function may be linked to telomere regulation. ZNF146 is strongly overexpressed in many pancreas and colorectal cancers. Increased gene copy numbers are detected in 3 of 12 tumor cell lines and 2 of 12 primary pancreatic carcinomas. ZNF146 is overexpresseed in 80% of colorectal cancers.1) Antoine, K., et al. J. Cell. Biochem. 95(4):763-768(2005) |
| Immunogen | This ZNF146 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-45 amino acids from the N-terminal region of human ZNF146. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ZNF146 |
|---|---|
| Alternative Names | Zinc finger protein OZF Only zinc finger protein Zinc finger protein 146 ZNF146 OZF |
| Molecular Weight(MW) | 33 kDa |
Application
Western blot analysis in K562 cell line lysates (35ug/lane).
Application
Western blot analysis in mouse heart tissue lysates (35ug/lane).
Application
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
