ZNF282 contains 1 KRAB domain and 5 C2H2-type zinc fingers. It belongs to the krueppel C2H2-type zinc-finger protein family and binds to the U5 repressive element (U5RE) of the human T cell leukemia virus type I long terminal repeat.1) Cheng, J., (2005) Science 308 (5725), 1149-1154.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ZNF282.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
ZNF282 antibody can be used for detection of ZNF282 by ELISA at 1:312500. ZNF282 antibody can be used for detection of ZNF282 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Transfected 293T at 1.25 ug/ml.
Positive Control
1) Tranfected 293T Cell Lysate
Application Notes
ZNF282 antibody can be used for detection of ZNF282 by ELISA at 1:312500. ZNF282 antibody can be used for detection of ZNF282 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store ZNF282 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt