Application
Antibody used in WB on Transfected 293T at 1.25 ug/ml.Application
Antibody used in WB on Bewo c, HEK, JEG3, PC3, SHEP at: 1:1000 (Lane 1: 20ug Bewo cells Lane 2: 20ug HEK cells Lane 3: 20ug JEG3 cells Lane 4: 20ug PC3 cells Lane 5: 20ug SHEP cells ).| Product Name | ZNF282 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ZNF282, HUB1 |
| Product description | ZNF282 contains 1 KRAB domain and 5 C2H2-type zinc fingers. It belongs to the krueppel C2H2-type zinc-finger protein family and binds to the U5 repressive element (U5RE) of the human T cell leukemia virus type I long terminal repeat.1) Cheng, J., (2005) Science 308 (5725), 1149-1154. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ZNF282. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ZNF282 antibody can be used for detection of ZNF282 by ELISA at 1:312500. ZNF282 antibody can be used for detection of ZNF282 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ZNF282 |
|---|---|
| Alternative Names | ZNF282 HUB1 |
| Molecular Weight(MW) | 74 kDa |
Application
Antibody used in WB on Transfected 293T at 1.25 ug/ml.
Application
Antibody used in WB on Bewo c, HEK, JEG3, PC3, SHEP at: 1:1000 (Lane 1: 20ug Bewo cells Lane 2: 20ug HEK cells Lane 3: 20ug JEG3 cells Lane 4: 20ug PC3 cells Lane 5: 20ug SHEP cells ).| Positive Control | 1) Tranfected 293T Cell Lysate |
|---|---|
| Application Notes | ZNF282 antibody can be used for detection of ZNF282 by ELISA at 1:312500. ZNF282 antibody can be used for detection of ZNF282 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store ZNF282 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
