Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.| Product Name | ZNF446 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ZNF446, ZSCAN30, ZSCAN52, ZKSCAN20 |
| Product description | ZNF446 contains a KRAB and three C (2)H (2) zinc fingers. ZNF446 is a transcription repressor when fused to GAL4 DNA-binding domain and co-transfected with VP-16. Overexpression of ZNF446 in COS-7 cells inhibits the transcriptional activities of SRE and AP-1, suggesting that the ZNF446 protein may act as a transcriptional repressor in mitogen-activated protein kinase (MAPK) signaling pathway to mediate cellular functions.1) Liu, F., et al., (2005) Biochem. Biophys. Res. Commun. 333 (1), 5-13. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ZNF446. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ZNF446 antibody can be used for detection of ZNF446 by ELISA at 1:1562500. ZNF446 antibody can be used for detection of ZNF446 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ZNF446 |
|---|---|
| Alternative Names | ZNF446 ZSCAN30 ZSCAN52 ZKSCAN20 |
| Molecular Weight(MW) | 49 kDa |
Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | ZNF446 antibody can be used for detection of ZNF446 by ELISA at 1:1562500. ZNF446 antibody can be used for detection of ZNF446 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store ZNF446 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
