Application
Western blot analysis of ZNF521 in HeLa cell lysate with ZNF521 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.Application
Immunofluorescence of ZNF521 in HeLa cells with ZNF521 antibody at 20 μg/mL.Application
Immunocytochemistry of ZNF521 in HeLa cells with ZNF521 antibody at 2.5 μg/mL.| Product Name | ZNF521 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | ZNF521 Antibody: The zinc finger protein 521 (ZNF521) is a transcription factor containing an N-terminal transcriptional repressor motif and 30 zinc finger domains. It plays a role in both erythroid cell and osteoblast differentiation during development, inhibiting the activities of early B-cell factor 1 (EBF1) in erythroid cells and Runx2 in osteoblast precursors. ZFP521 binds to both Runx2 and histone deacetylase 3 (HDAC3), promotes their association and antagonizes Runx2 transcriptional activity in a HDAC3-dependent manner, thereby regulating osteoblast differentiation, skeletal development, and bone homeostasis.1) Warming S, Liu P, Suzuki T, et al. Evi3, a common retroviral integration site in murine B-cell lymphoma, encodes a EBFAZ-related Kruppel-like zinc finger protein. Blood 2003; 101:1934-40. |
| Immunogen | ZNF521 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human ZNF521. The immunogen is located within the first 50 amino acids of ZNF521. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| ZNF521 antibody can be used for detection of ZNF521 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ZNF521 |
|---|---|
| Alternative Names | ZNF521 Antibody: EHZF Evi3 EHZF LIP3 Zinc finger protein 521 Early hematopoietic zinc finger protein |
| Molecular Weight(MW) | Predicted: 144 kDa Observed: 145 kDa |
| Sequence Similarities | 2) Cat. No. 17-001 - HeLa Cell Slide |
| Tissue Specificity | ZNF521 antibody is human specific. |
Application
Western blot analysis of ZNF521 in HeLa cell lysate with ZNF521 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Application
Immunofluorescence of ZNF521 in HeLa cells with ZNF521 antibody at 20 μg/mL.
Application
Immunocytochemistry of ZNF521 in HeLa cells with ZNF521 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | ZNF521 antibody can be used for detection of ZNF521 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | ZNF521 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ZNF521 Antibody is supplied in PBS containing 0.02% sodium azide. |
