Application
Western blot analysis of ZNF821 in Jurkat cell lysate with ZNF821 antibody at 0.5 μg/ml in (A) the absence and (B) the presence of blocking peptide.Application
Immunofluorescence ofZNF821 in Jurkat cells with ZNF821 antibody at 20 μg/mL.Application
Immunocytochemistry of ZNF821 in Jurkat cells with ZNF821 antibody at 2.5 μg/mL.| Product Name | ZNF821 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ZNF821 Antibody: Zinc finger protein 821 |
| Product description | The ZNF821 protein contains two C2H2 zinc finger motifs and a score-and-three (23)-amino acid peptide repeat (STPR) domain. The STPR domain of the encoded protein binds to double stranded DNA and may also contain a nuclear localization signal, suggesting that this protein interacts with chromosomal DNA (1). The exact function of ZNF821, however, remains unknown.1) Nonaka Y, Muto H, Aizawa T, et al. STPR, a 23-amino acid tandem repeat domain, found in the human function-unknown protein ZNF821. Biochemistry 2010; 49:8367-75. |
| Immunogen | ZNF821 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human ZNF821. The immunogen is located within amino acids 270 - 320 of ZNF821. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| ZNF821 antibody can be used for detection of ZNF821 by Western blot at 0.5 - 1 μg/ml. Antibody can also be used for Immunocytochemistry at 2.5 μg/mL. For Immunoflorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ZNF821 |
|---|---|
| Alternative Names | ZNF821 Antibody: Zinc finger protein 821 |
| Molecular Weight(MW) | Predicted: 41 kDa Observed: 38 kDa |
| Sequence Similarities | 2) Cat. No. 17-005 - Jurkat Cell Slide |
| Tissue Specificity | ZNF821 antibody is human and mouse reactive. At least three isoforms of ZNF821 are known to exist; this antibody will detect the two largest isoforms. |
| Entrez Gene | 55565 |
|---|---|
| Protein Accession | NP_001188482.1 |
Application
Western blot analysis of ZNF821 in Jurkat cell lysate with ZNF821 antibody at 0.5 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Application
Immunofluorescence ofZNF821 in Jurkat cells with ZNF821 antibody at 20 μg/mL.
Application
Immunocytochemistry of ZNF821 in Jurkat cells with ZNF821 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | ZNF821 antibody can be used for detection of ZNF821 by Western blot at 0.5 - 1 μg/ml. Antibody can also be used for Immunocytochemistry at 2.5 μg/mL. For Immunoflorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ZNF821 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | ZNF821 antibody is supplied in PBS containing 0.02% sodium azide. |
