Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.Application
Antibody used in WB on recombinant human ZP4 protein.| Product Name | ZP4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ZP4, ZBP, ZP1, ZPB, Zp-4 |
| Product description | The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix.The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a consensus furin cleavage site, and a C-terminal transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. Previously, this gene has been referred to as ZP1 or ZPB and thought to have similar functions as mouse Zp1. However, a human gene with higher similarity and chromosomal synteny to mouse Zp1 has been assigned the symbol ZP1 and this gene has been assigned the symbol ZP4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.1) Gregory, S.G., (2006) Nature 441 (7091), 315-321. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ZP4. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ZP4 antibody can be used for detection of ZP4 by ELISA at 1:1562500. ZP4 antibody can be used for detection of ZP4 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ZP4 |
|---|---|
| Alternative Names | ZP4 ZBP ZP1 ZPB Zp-4 |
| Molecular Weight(MW) | 49 kDa |
Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Application
Antibody used in WB on recombinant human ZP4 protein.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | ZP4 antibody can be used for detection of ZP4 by ELISA at 1:1562500. ZP4 antibody can be used for detection of ZP4 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store ZP4 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
