Application
Western Blot at 1:1000 dilution + MOLT-4 whole cell lysate Lysates/proteins at 20 ug per lane.Application
Western blot analysis of ZWINT antibody in mouse cerebellum tissue lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with ZWINT Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Flow cytometric analysis of widr cells using ZWINT Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of ZWINT using rabbit polyclonal ZWINT Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ZWINT gene.| Product Name | ZWINT Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ZW10 interactor, ZW10-interacting protein 1, Zwint-1, ZWINT |
| Product description | ZWINT is clearly involved in kinetochore function although an exact role is not known. It interacts with ZW10, another kinetochore protein, possibly regulating the association between ZW10 and kinetochores. The protein localizes to prophase kinetochores before ZW10 does and it remains detectable on the kinetochore until late anaphase. It has a uniform distribution in the cytoplasm of interphase cells.1) Famulski,J.K., J. Cell Biol. 180 (3), 507-520 (2008) Kops,G.J., J. Cell Biol. 169 (1), 49-60 (2005) Wang,H., J. Biol. Chem. 279 (52), 54590-54598 (2004) |
| Immunogen | This ZWINT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59-88 amino acids from the Central region of human ZWINT. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | ZWINT |
|---|---|
| Alternative Names | ZW10 interactor ZW10-interacting protein 1 Zwint-1 ZWINT |
| Molecular Weight(MW) | 31 kDa |
Application
Western Blot at 1:1000 dilution + MOLT-4 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Western blot analysis of ZWINT antibody in mouse cerebellum tissue lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with ZWINT Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Flow cytometric analysis of widr cells using ZWINT Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of ZWINT using rabbit polyclonal ZWINT Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ZWINT gene.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
