| Product Name | Rabbit Anti-Chicken IgY (H&L) Antibody (Alkaline Phosphatase) |
|---|---|
| Antibody Type | Secondary Antibodies |
| Immunogen | Chicken IgY - whole molecule |
| Conjugation | Alkaline Phosphatase |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgY |
| Host Species | Rabbit |
| Tested Applications | ELISA |
| Alkaline Phosphatase Conjugated Affinity Purified anti-Chicken IgY is suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. This product has been assayed against 1.0 mg of Chicken IgY in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:12,000 is suggested for this product. (Optimal dilution should be determined by user).: |
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| Species Reactivity | Chicken |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target Description | Reacts with the heavy chain of chicken IgY. |
|---|
| Application Notes | Alkaline Phosphatase Conjugated Affinity Purified anti-Chicken IgY is suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. This product has been assayed against 1.0 mg of Chicken IgY in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:12,000 is suggested for this product. (Optimal dilution should be determined by user).: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Alkaline Phosphatase Conjugated Affinity Purified anti-Chicken IgY can be stored vial at 4 ˚C before opening. DO NOT FREEZE. This product is stable at 4 ˚C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening product. |
| Storage Buffer | 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0; 0.1% (w/v) Sodium Azide; 10 mg/mL Bovine Serum Albumin (BSA) |
