Application
Fig1: Western blot analysis of CD4 on mouse brain lysate using anti-CD4 antibody at 1/500 dilution.Application
Fig2: ICC staining CD4 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Flow cytometric analysis of Raji cells with CD4 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | CD4 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD 4 antibody CD4 (L3T4) antibody CD4 antibody CD4 antigen (p55) antibody CD4 antigen antibody CD4 molecule antibody CD4 receptor antibody CD4+ Lymphocyte deficiency, included antibody CD4_HUMAN antibody CD4mut antibody L3T4 antibody Leu3 antibody Ly-4 antibody Lymphocyte antigen CD4 antibody MGC165891 antibody OTTHUMP00000238897 antibody p55 antibody T cell antigen T4 antibody T cell antigen T4/LEU3 antibody T cell differentiation antigen L3T4 antibody T cell OKT4 deficiency, included antibody T cell surface antigen T4/Leu 3 antibody T cell surface antigen T4/Leu3 antibody T cell surface glycoprotein CD4 antibody T-cell surface antigen T4/Leu-3 antibody T-cell surface glycoprotein CD4 antibody W3/25 antibody W3/25 antigen antibody |
| Product description | The CD4 gene encodes the T4 antigen, a glycoprotein of approximately 55 kDa, expressed on the surface of helper T lymphocytes.CD4 antigens are members of the immunoglobulin supergene family and are implicated as associative recognition elements in major histocompatibility complex class II-restricted immune responses. On T-lymphocytes they define the helper/inducer subset. CD4 antigens also serve as interleukin-15 receptors and bind to the HIV receptors, binding directly to the HIV envelope protein GP120. |
| Immunogen | This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to mouse CD4 aa 200-250. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCFC |
| WB:1:500 ICC |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | CD 4 antibody
CD4 (L3T4) antibody
CD4 antibody
CD4 antigen (p55) antibody
CD4 antigen antibody
CD4 molecule antibody
CD4 receptor antibody
CD4+ Lymphocyte deficiency included antibody CD4_HUMAN antibody CD4mut antibody L3T4 antibody Leu3 antibody Ly-4 antibody Lymphocyte antigen CD4 antibody MGC165891 antibody OTTHUMP00000238897 antibody p55 antibody T cell antigen T4 antibody T cell antigen T4/LEU3 antibody T cell differentiation antigen L3T4 antibody T cell OKT4 deficiency included antibody T cell surface antigen T4/Leu 3 antibody T cell surface antigen T4/Leu3 antibody T cell surface glycoprotein CD4 antibody T-cell surface antigen T4/Leu-3 antibody T-cell surface glycoprotein CD4 antibody W3/25 antibody W3/25 antigen antibody |
|---|---|
| Molecular Weight(MW) | 51kDa |
| Cellular Localization | Cell Membrane |
Application
Fig1: Western blot analysis of CD4 on mouse brain lysate using anti-CD4 antibody at 1/500 dilution.
Application
Fig2: ICC staining CD4 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Flow cytometric analysis of Raji cells with CD4 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | Mouse brain tissue, LOVO, Raji. |
|---|---|
| Application Notes | WB:1:500 ICC |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 25%Glycerol. Preservative: 0.05% Sodium Azide. |
