Application
Fig1: Western blot analysis of Cytokeratin 18 on mouse liver tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.Application
Fig2: ICC staining Cytokeratin 18 in 293T cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Cytokeratin 18 polyclonal antibody at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. AlexaFluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).Application
Fig3: ICC staining Cytokeratin 18 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Cytokeratin 18 polyclonal antibody at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. AlexaFluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).Application
Fig4: ICC staining Cytokeratin 18 in SW480 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Cytokeratin 18 polyclonal antibody at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. AlexaFluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Cytokeratin 18 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with 0407-1 at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Cytokeratin 18 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with 0407-1 at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.| Product Name | Cytokeratin 18 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cell proliferation inducing gene 46 protein antibody Cell proliferation inducing protein 46 antibody Cell proliferation-inducing gene 46 protein antibody CK 18 antibody CK-18 antibody CK18 antibody CYK 18 antibody CYK18 antibody Cytokeratin 18 antibody Cytokeratin endo B antibody Cytokeratin-18 antibody K 18 antibody K18 antibody K1C18_HUMAN antibody KA18 antibody Keratin 18 antibody Keratin 18, type I antibody Keratin D antibody keratin, type I cytoskeletal 18 antibody Keratin-18 antibody Krt18 antibody |
| Product description | Cytokeratin 18 is an acidic keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only Cytokeratins 8 and 18. |
| Immunogen | This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to a region of C-terminal residues of mouse CK-18. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHC |
| WB 1:25,000-50,000: ICC 1:200: IHC 1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Cell proliferation inducing gene 46 protein antibody
Cell proliferation inducing protein 46 antibody
Cell proliferation-inducing gene 46 protein antibody
CK 18 antibody
CK-18 antibody
CK18 antibody
CYK 18 antibody
CYK18 antibody
Cytokeratin 18 antibody
Cytokeratin endo B antibody
Cytokeratin-18 antibody
K 18 antibody
K18 antibody
K1C18_HUMAN antibody
KA18 antibody
Keratin 18 antibody
Keratin 18 type I antibody Keratin D antibody keratin type I cytoskeletal 18 antibody Keratin-18 antibody Krt18 antibody |
|---|---|
| Molecular Weight(MW) | 48kDa |
| Cellular Localization | Cytoplasm, perinuclear region |
Application
Fig1: Western blot analysis of Cytokeratin 18 on mouse liver tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Fig2: ICC staining Cytokeratin 18 in 293T cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Cytokeratin 18 polyclonal antibody at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. AlexaFluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
Application
Fig3: ICC staining Cytokeratin 18 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Cytokeratin 18 polyclonal antibody at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. AlexaFluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
Application
Fig4: ICC staining Cytokeratin 18 in SW480 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Cytokeratin 18 polyclonal antibody at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. AlexaFluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Cytokeratin 18 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with 0407-1 at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Cytokeratin 18 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with 0407-1 at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.| Positive Control | Mouse liver tissue, 293T, A549, SW480, mouse kidney tissue. |
|---|---|
| Application Notes | WB 1:25,000-50,000: ICC 1:200: IHC 1:100: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 25%Glycerol. Preservative: 0.05% Sodium Azide. |
