Application
Fig1: Western blot analysis of Synaptophysin on different lysates using anti-Synaptophysin antibody at 1/1,000 dilution.Application
Fig2: ICC staining Synaptophysin in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using anti-Synaptophysin antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Synaptophysin antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of SHG-44 cells with Synaptophysin antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | Synaptophysin |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Major synaptic vesicle protein p38 antibody MRX96 antibody MRXSYP antibody Syn p38 antibody Synaptophysin antibody Syp antibody SYPH antibody SYPH_HUMAN antibody SypI antibody |
| Product description | Synaptophysin is a 38 kDa glycoprotein present in the membrane of neuronal presynaptic vesicles in brain, spinal cord, retina, vesicles of adrenal medulla, neuromuscular junctions, and endocrine cells. It is also expressed by neuroendocrine cells throughout the body, both normal and neoplastic. Synaptophysin is a useful marker for the identification of normal neuroendocrine cells and neuroendocrine neoplasms. |
| Immunogen | Synthetic peptide (KLH-coupled) within human Synaptophysin 220-280 aa. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:500~1:2000 ICC:1:200~1:500 IHC:1:50~1:200 FC:1:200~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Major synaptic vesicle protein p38 antibody MRX96 antibody MRXSYP antibody Syn p38 antibody Synaptophysin antibody Syp antibody SYPH antibody SYPH_HUMAN antibody SypI antibody |
|---|---|
| Molecular Weight(MW) | 34kDa |
| Cellular Localization | Cytoplasmic vesicle. Membrane. Synapse. Synaptosome. |
Application
Fig1: Western blot analysis of Synaptophysin on different lysates using anti-Synaptophysin antibody at 1/1,000 dilution.
Application
Fig2: ICC staining Synaptophysin in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using anti-Synaptophysin antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Synaptophysin antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of SHG-44 cells with Synaptophysin antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | Human brain tissue, mouse brain tissue, N2A, rat pancreas tissue, SHG-44. |
|---|---|
| Application Notes | WB:1:500~1:2000 ICC:1:200~1:500 IHC:1:50~1:200 FC:1:200~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 25%Glycerol. Preservative: 0.05% Sodium Azide. |
