Application
Fig1: Western blot analysis of ALP on different cell lysates using anti-ALP antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: HepG2 Lane 3: MG-63Application
Fig2: ICC staining ALP in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining ALP in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining ALP in SW480 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-ALP antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-ALP antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ALP antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ALP antibody. Counter stained with hematoxylin.Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-ALP antibody. Counter stained with hematoxylin.Application
Fig10: Flow cytometric analysis of Hela cells with ALP antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Product Name | Alkaline Phosphatase [SA40-00] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Alkaline phosphatase antibody Alkaline phosphatase placental antibody Alkaline phosphatase placental type antibody Alkaline phosphatase Regan isozyme antibody ALP antibody Alp1 antibody ALPP antibody FLJ61142 antibody Germ-cell alkaline phosphatase antibody nagao isozyme antibody OTTHUMP00000164354 antibody PALP antibody Placental alkaline phosphatase 1 antibody placental heat-stable alkaline phosphatase antibody placental type antibody PLAP antibody PLAP-1 antibody PLAP1 antibody PPB1_HUMAN antibody |
| Product description | Alkaline phosphatase (ALP) removes phosphate groups from the 5' end of DNA and RNA, and from proteins. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. Placental ALP is highly polymorphic, there are at least three common alleles. Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIHCIPFC |
| WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:10-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Alkaline phosphatase antibody Alkaline phosphatase placental antibody Alkaline phosphatase placental type antibody Alkaline phosphatase Regan isozyme antibody ALP antibody Alp1 antibody ALPP antibody FLJ61142 antibody Germ-cell alkaline phosphatase antibody nagao isozyme antibody OTTHUMP00000164354 antibody PALP antibody Placental alkaline phosphatase 1 antibody placental heat-stable alkaline phosphatase antibody placental type antibody PLAP antibody PLAP-1 antibody PLAP1 antibody PPB1_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 57 kDa |
| Cellular Localization | Cell membrane |
Application
Fig1: Western blot analysis of ALP on different cell lysates using anti-ALP antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: HepG2 Lane 3: MG-63
Application
Fig2: ICC staining ALP in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining ALP in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining ALP in SW480 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-ALP antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-ALP antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ALP antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ALP antibody. Counter stained with hematoxylin.
Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-ALP antibody. Counter stained with hematoxylin.
Application
Fig10: Flow cytometric analysis of Hela cells with ALP antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Positive Control | SW480, MG-63, Hela, HepG2, mouse kidney tissue, human liver tissue, mouse small intestine tissue, human liver cancer tissue, human kidney tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:10-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
