Application
Fig1: Western blot analysis of VCP on different cell lysates using anti- VCP antibody at 1/2000 dilution. Positive control: Lane 1: L929 Lane 2: F9 Lane 3: A549 Lane 4: MCF-7 Lane 5: Hela Lane 6: NIH/3T3 Lane 7: N2A Lane 8: Jurkat Lane 9: HepG2 Lane 10: A431 Lane 11: Mouse heart Lane 12: Mouse brain Lane 13: Mouse liver Lane 14: Mouse kidneyApplication
Fig2: ICC staining VCP in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining VCP in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining VCP in SHG-44 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: ICC staining VCP in SW480 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-VCP antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-VCP antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-VCP antibody. Counter stained with hematoxylin.| Product Name | VCP |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 15S Mg(2+) ATPase p97 subunit antibody 15S Mg(2+)-ATPase p97 subunit antibody ALS14 antibody ATPase p97 antibody CDC48 antibody IBMPFD antibody MGC131997 antibody MGC148092 antibody MGC8560 antibody p97 antibody TER ATPase antibody TERA antibody TERA_HUMAN antibody Transitional endoplasmic reticulum ATPase antibody Valosin containing protein antibody Valosin-containing protein antibody VCP antibody Yeast Cdc48p homolog antibody |
| Product description | Valosin-containing protein (VCP) is a highly conserved, that belongs to the AAA (ATPase associated with a variety of cellular activities) family of proteins. It is necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. VCP is involved in the formation of the transitional endoplasmic reticulum (tER). VCP homo-hexamers associate with a variety of protein cofactors to form many distinct protein complexes, which act as chaperones to unfold proteins and transport them to specific cellular compartments or to the proteosome. |
| Immunogen | peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHC |
| WB:1:2,000 ICC:1:200 IHC:1:200 |
|
| Species Reactivity | HumanMouseZebra Fish |
| Concentration | 1mg/ml |
| Alternative Names | 15S Mg(2+) ATPase p97 subunit antibody 15S Mg(2+)-ATPase p97 subunit antibody ALS14 antibody ATPase p97 antibody CDC48 antibody IBMPFD antibody MGC131997 antibody MGC148092 antibody MGC8560 antibody p97 antibody TER ATPase antibody TERA antibody TERA_HUMAN antibody Transitional endoplasmic reticulum ATPase antibody Valosin containing protein antibody Valosin-containing protein antibody VCP antibody Yeast Cdc48p homolog antibody |
|---|---|
| Molecular Weight(MW) | 89 kDa |
| Cellular Localization | Cytoplasm, nucleus, endoplasmic reticulum |
| SwissProt ID | P55072 |
|---|
Application
Fig1: Western blot analysis of VCP on different cell lysates using anti- VCP antibody at 1/2000 dilution. Positive control: Lane 1: L929 Lane 2: F9 Lane 3: A549 Lane 4: MCF-7 Lane 5: Hela Lane 6: NIH/3T3 Lane 7: N2A Lane 8: Jurkat Lane 9: HepG2 Lane 10: A431 Lane 11: Mouse heart Lane 12: Mouse brain Lane 13: Mouse liver Lane 14: Mouse kidney
Application
Fig2: ICC staining VCP in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining VCP in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining VCP in SHG-44 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: ICC staining VCP in SW480 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-VCP antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-VCP antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-VCP antibody. Counter stained with hematoxylin.| Positive Control | L929, F9, SW480, A549, MCF-7, Hela, NIH/3T3, N2A, Jurkat, HepG2, A431,mouse brain tissue, mouse heart tissue, mouse liver tissue, mouse kidney tissue,human uterus tissue,SHG-44 cells ,mouse skeletal muscle tissue . |
|---|---|
| Application Notes | WB:1:2,000 ICC:1:200 IHC:1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
