Application
Fig1: Western blot analysis of Tie-1 on K562 cell lysate using anti-Tie-1 antibody at 1/2,000 dilution.Application
Fig2: ICC staining Tie-1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Tie-1 in SW620 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Product Name | TIE-1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | JKT 14 antibody JTK14 antibody TIE antibody Tie1 antibody TIE1_HUMAN antibody Tyrosine kinase with immunoglobulin and epidermal growth factor homology domains 1 antibody Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains antibody Tyrosine kinase with immunoglobulin-like and EGF-like domains 1 antibody Tyrosine protein kinase receptor Tie 1 antibody Tyrosine-protein kinase receptor Tie-1 antibody |
| Product description | The Tie-1 receptor is an endothelial specific cell surface tyrosine kinase that is indispensable for endothelial functions. Human TIE1 cDNA encodes a 1138 amino acid residue precursor protein with a putative signal peptide, an extracellular domain, and a cytoplasmic domain. Human TIE1/Fc, a disulfide linked homodimeric protein, has a calculated molecular mass of approximately 107 kDa. Tie-1 and Tie-2 are receptor tyrosine kinases (RTKs) that are exclusively expressed in endothelial cells and play important roles in endothelial cell biology. |
| Immunogen | Synthetic peptide within human TIE-1 40-120aa. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICC |
| WB:1:1,000-1:5,000 ICC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | JKT 14 antibody JTK14 antibody TIE antibody Tie1 antibody TIE1_HUMAN antibody Tyrosine kinase with immunoglobulin and epidermal growth factor homology domains 1 antibody Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains antibody Tyrosine kinase with immunoglobulin-like and EGF-like domains 1 antibody Tyrosine protein kinase receptor Tie 1 antibody Tyrosine-protein kinase receptor Tie-1 antibody |
|---|---|
| Molecular Weight(MW) | 125kDa |
| Cellular Localization | Cell membrane. Membrane. |
| SwissProt ID | P35590 |
|---|
Application
Fig1: Western blot analysis of Tie-1 on K562 cell lysate using anti-Tie-1 antibody at 1/2,000 dilution.
Application
Fig2: ICC staining Tie-1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Tie-1 in SW620 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Positive Control | K562, HepG2, SW620. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 25%Glycerol. Preservative: 0.05% Sodium Azide. |
