Application
Fig1: Western blot analysis of CREB on different cell lysates using anti-CREB antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: HepG2 Lane 3: Jurkat Lane 4: NIH/3T3Application
Fig2: ICC staining CREB in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CREB antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-CREB antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-CREB antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CREB antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of Hela cells with CREB antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.Application
Fig8: Western blot analysis of CREB on Zebrafish tissue lysates using anti-CREB antibody at 1/1,000 dilution.| Product Name | CREB [SA04-04] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Active transcription factor CREB antibody cAMP response element binding protein 1 antibody cAMP response element binding protein antibody cAMP responsive element binding protein 1 antibody cAMP-responsive element-binding protein 1 antibody CREB antibody CREB-1 antibody CREB1 antibody CREB1_HUMAN antibody Cyclic AMP-responsive element-binding protein 1 antibody MGC9284 antibody OTTHUMP00000163864 antibody OTTHUMP00000163865 antibody OTTHUMP00000206660 antibody OTTHUMP00000206662 antibody OTTHUMP00000206667 antibody Transactivator protein antibody |
| Product description | Eukaryotic gene transcription is regulated by sequence-specific transcription factors that bind modular cis-acting promoter and enhancer elements. The ATF/CREB transcription factor family binds the palindromic cAMP response element (CRE) octanucleotide TGACGTCA. The ATF/CREB family includes CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3. This family of proteins contains highly divergent N-terminal domains, but shares a C-terminal leucine zipper for dimerization and DNA binding. Although CREB can bind to DNA in an unphosphorylated state, it cannot activate transcription. Phosphorylation of CREB on Ser 133 by protein kinase A facilitates its interaction with the CREB-binding protein (CBP) and activates the basal transcription complex. CREB functions in neoglucogenesis through interactions with the nuclear coactivator PGC-1. CREB may play a role in the pathogenesis of type II diabetes and dilated cardiomyopathy. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:10-1:100 |
|
| Species Reactivity | HumanMouseRatZebra Fish |
| Concentration | 1mg/ml |
| Alternative Names | Active transcription factor CREB antibody cAMP response element binding protein 1 antibody cAMP response element binding protein antibody cAMP responsive element binding protein 1 antibody cAMP-responsive element-binding protein 1 antibody CREB antibody CREB-1 antibody CREB1 antibody CREB1_HUMAN antibody Cyclic AMP-responsive element-binding protein 1 antibody MGC9284 antibody OTTHUMP00000163864 antibody OTTHUMP00000163865 antibody OTTHUMP00000206660 antibody OTTHUMP00000206662 antibody OTTHUMP00000206667 antibody Transactivator protein antibody |
|---|---|
| Molecular Weight(MW) | 40 kDa |
| Cellular Localization | Nucleus |
Application
Fig1: Western blot analysis of CREB on different cell lysates using anti-CREB antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: HepG2 Lane 3: Jurkat Lane 4: NIH/3T3
Application
Fig2: ICC staining CREB in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CREB antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-CREB antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-CREB antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CREB antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of Hela cells with CREB antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Application
Fig8: Western blot analysis of CREB on Zebrafish tissue lysates using anti-CREB antibody at 1/1,000 dilution.| Positive Control | Jurkat, NIH/3T3, Hela, HepG2, human tonsil tissue, human lung cancer tissue, mouse brain tissue, human breast carcinoma tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:10-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
