Application
Fig1: Western blot analysis of Noggin on MG-63 cell lysate using anti-Noggin antibody at 1/1,000 dilution.Application
Fig2: ICC staining Noggin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Noggin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Noggin antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Noggin antibody. Counter stained with hematoxylin.| Product Name | Noggin |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Nog antibody NOGG_HUMAN antibody Noggin antibody SYM 1 antibody SYM1 antibody Symphalangism 1 (proximal) antibody Synostoses (multiple) syndrome 1 antibody SYNS 1 antibody SYNS1 antibody |
| Product description | Noggin is a bone morphogenetic protein (BMP) antagonist expressed in Spemann's organizer. Murine Noggin is expressed in condensing cartilage and immature chondrocytes, as are many BMPs. It is essential for cartilage morphogenesis and joint formation. Defects in NOG are the cause of multiple synostoses syndrome 1 (SYNS1), symphalangism proximal syndrome (SYM1) and tarsal-carpal coalition syndrome (TCC). |
| Immunogen | This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to a region of Noggin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHC |
| WB:1:500-1:1,000 ICC |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Nog antibody NOGG_HUMAN antibody Noggin antibody SYM 1 antibody SYM1 antibody Symphalangism 1 (proximal) antibody Synostoses (multiple) syndrome 1 antibody SYNS 1 antibody SYNS1 antibody |
|---|---|
| Molecular Weight(MW) | 26kDa |
| Cellular Localization | Secreted. |
Application
Fig1: Western blot analysis of Noggin on MG-63 cell lysate using anti-Noggin antibody at 1/1,000 dilution.
Application
Fig2: ICC staining Noggin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Noggin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Noggin antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Noggin antibody. Counter stained with hematoxylin.| Positive Control | MG-63, human brain, mouse brain |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
