Application
Fig1: Western blot analysis of Filamin A on different cell lysates using anti-Filamin A antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7 Lane 2: Jurkat Lane 3: Hela Lane 4: NIH/3T3Application
Fig2: ICC staining Filamin A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Filamin A in Ags cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining Filamin A in HUVEC cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-Filamin A antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse uterus tissue using anti-Filamin A antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of Hela cells with Filamin A antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | Filamin A [SA30-08] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ABP 280 antibody ABP-280 antibody Actin-binding protein 280 antibody Alpha filamin antibody Alpha-filamin antibody APBX antibody CSBS antibody CVD1 antibody Endothelial actin binding protein antibody Endothelial actin-binding protein antibody Filamin 1 antibody Filamin A alpha antibody Filamin A antibody Filamin-1 antibody Filamin-A antibody FLN antibody FLN-A antibody FLN1 antibody FLNA antibody FLNA_HUMAN antibody FMD antibody MNS antibody NHBP antibody Non muscle filamin antibody Non-muscle filamin antibody OPD antibody OPD1 antibody OPD2 antibody XLVD antibody XMVD antibody |
| Product description | Caldesmon, Filamin 1, Nebulin and Villin are differentially expressed and regulated Actin binding proteins. Both muscular (CDh) and non-muscular (CDl) forms of Caldesmon have been identified and each has been shown to bind to Actin as well as to calmodulin and Myosin. CDh is expressed predominantly on thin filaments in smooth muscle, whereas CDl is widely expressed in non-muscle tissues and cells. Filamin 1, which is ubiquitously expressed and exists as a homodimer, functions to crosslink Actin to filaments. Nebulin is a large filamentous protein specific to muscle tissue that may function as a ruler for filament length. Several isoforms of Nebulin are produced by alternative exon usage. Villin is Ca2;+;-regulated and is the major structural component of the brush border of absorptive cells. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCFC |
| WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:400 FC:1:10-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | ABP 280 antibody ABP-280 antibody Actin-binding protein 280 antibody Alpha filamin antibody Alpha-filamin antibody APBX antibody CSBS antibody CVD1 antibody Endothelial actin binding protein antibody Endothelial actin-binding protein antibody Filamin 1 antibody Filamin A alpha antibody Filamin A antibody Filamin-1 antibody Filamin-A antibody FLN antibody FLN-A antibody FLN1 antibody FLNA antibody FLNA_HUMAN antibody FMD antibody MNS antibody NHBP antibody Non muscle filamin antibody Non-muscle filamin antibody OPD antibody OPD1 antibody OPD2 antibody XLVD antibody XMVD antibody |
|---|---|
| Molecular Weight(MW) | 281 kDa |
| Cellular Localization | Cytoplasm |
Application
Fig1: Western blot analysis of Filamin A on different cell lysates using anti-Filamin A antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7 Lane 2: Jurkat Lane 3: Hela Lane 4: NIH/3T3
Application
Fig2: ICC staining Filamin A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Filamin A in Ags cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Filamin A in HUVEC cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-Filamin A antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse uterus tissue using anti-Filamin A antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of Hela cells with Filamin A antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | HUVEC, MCF-7, Hela, Ags, Jurkat, NIH/3T3, mouse uterus tissue, human uterus tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:400 FC:1:10-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
