Application
Fig1: Western blot analysis of NCAML1 on human fetal brain tissue lysate using anti-NCAML1 antibody at 1/1,000 dilution.Application
Fig2: ICC staining NCAML1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining NCAML1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-NCAML1 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-NCAML1 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-NCAML1 antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of SHG-44 cells with NCAML1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | NCAM-L1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Antigen identified by monoclonal antibody R1 antibody CAML1 antibody CD171 antibody CD171 antigen antibody HSAS antibody HSAS1 antibody Hyd antibody L1 antibody L1 cell adhesion molecule antibody L1-NCAM antibody L1cam antibody L1CAM_HUMAN antibody MASA antibody MIC5 antibody N CAML1 antibody N-CAM-L1 antibody NCAM-L1 antibody NCAML1 antibody Nerve-growth factor-inducible large external glycoprotein antibody Neural cell adhesion molecule L1 antibody NILE antibody OTTHUMP00000025992 antibody S10 antibody SPG1 antibody |
| Product description | The neural cell adhesion molecule L1 (NCAM-L1) is an immunoglobulin superfamily adhesion molecule that is critical for neural development, involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. Defects in L1CAM are the cause of hydrocephalus due to stenosis of the aqueduct of Sylvius (HSAS). Hydrocephalus is a condition in which abnormal accumulation of cerebrospinal fluid in the brain causes increased intracranial pressure inside the skull. Defects in L1CAM are also the cause of mental retardation, aphasia, shuffling gait, and adducted thumbs syndrome (MASA), a X-linked disorder. |
| Immunogen | Synthetic peptide (KLH-coupled) corresponding to a region of C-terminal residues of NCAM-L1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Antigen identified by monoclonal antibody R1 antibody CAML1 antibody CD171 antibody CD171 antigen antibody HSAS antibody HSAS1 antibody Hyd antibody L1 antibody L1 cell adhesion molecule antibody L1-NCAM antibody L1cam antibody L1CAM_HUMAN antibody MASA antibody MIC5 antibody N CAML1 antibody N-CAM-L1 antibody NCAM-L1 antibody NCAML1 antibody Nerve-growth factor-inducible large external glycoprotein antibody Neural cell adhesion molecule L1 antibody NILE antibody OTTHUMP00000025992 antibody S10 antibody SPG1 antibody |
|---|---|
| Molecular Weight(MW) | 220kDa |
| Cellular Localization | Cell membrane. Cell projection. Membrane. |
Application
Fig1: Western blot analysis of NCAML1 on human fetal brain tissue lysate using anti-NCAML1 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining NCAML1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining NCAML1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-NCAML1 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-NCAML1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-NCAML1 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of SHG-44 cells with NCAML1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | Human fetal brain tissue, 293T, Hela, human breast cancer tissue, human kidney tissue, mouse kidney tissue, SHG-44. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
