Application
Fig1: Western blot analysis of CD62E on different cell lysates using anti-CD62E antibody at 1/500 dilution.Positive control：Lane1: MCF-7Lane2: RajiApplication
Fig2: ICC staining CD62E in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining CD62E in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining CD62E in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Flow cytometric analysis of Jurkat cells with CD62E antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | CD62E |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD26E antibody CD62 antigen-like family member E antibody CD62E antibody E selectin antibody E-selectin antibody ELAM antibody ELAM-1 antibody ELAM1 antibody Endothelial adhesion molecule 1 antibody Endothelial leukocyte adhesion molecule 1 antibody ESEL antibody LECAM2 antibody Leukocyte-endothelial cell adhesion molecule 2 antibody LYAM2_HUMAN antibody Sele antibody Selectin E antibody |
| Product description | CD62E, known as E-selectin, is a cell adhesion molecule expressed only on endothelial cells activated by cytokines. Like other selectins, it plays an important part in inflammation. During inflammation, E-selectin plays an important part in recruiting leukocytes to the site of injury. The main ligands recognised by CD62E are oligosaccharides related to sialyl lewis x. P-selectin has a similar function, but is expressed on the endothelial cell surface within minutes as it is stored within the cell rather than produced on demand. |
| Immunogen | This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to a region of N-terminal residues of CD62E. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCFC |
| WB:1:500 ICC:1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CD26E antibody CD62 antigen-like family member E antibody CD62E antibody E selectin antibody E-selectin antibody ELAM antibody ELAM-1 antibody ELAM1 antibody Endothelial adhesion molecule 1 antibody Endothelial leukocyte adhesion molecule 1 antibody ESEL antibody LECAM2 antibody Leukocyte-endothelial cell adhesion molecule 2 antibody LYAM2_HUMAN antibody Sele antibody Selectin E antibody |
|---|---|
| Molecular Weight(MW) | 67kDa |
| Cellular Localization | Cell membrane. Membrane. |
| SwissProt ID | P16581 |
|---|
Application
Fig1: Western blot analysis of CD62E on different cell lysates using anti-CD62E antibody at 1/500 dilution.Positive control：Lane1: MCF-7Lane2: Raji
Application
Fig2: ICC staining CD62E in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining CD62E in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining CD62E in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Flow cytometric analysis of Jurkat cells with CD62E antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | Hela, MCF-7, Raji, HepG2, Jurkat. |
|---|---|
| Application Notes | WB:1:500 ICC:1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
