Application
Fig1: Western blot analysis of HAPLN1 on different lysates using anti-HAPLN1 antibody at 1/1,000 dilution. Positive control: Lane 1: BT-20 Lane 2: HUVEC Lane 3: SW480Application
Fig2: ICC staining HAPLN1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining HAPLN1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining HAPLN1 in BT-20 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HAPLN1 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-HAPLN1 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-HAPLN1 antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-HAPLN1 antibody. Counter stained with hematoxylin.| Product Name | HAPLN1 [SY02-17] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cartilage link protein antibody cartilage linking protein 1 antibody Cartilage-link protein antibody Cartilage-linking protein 1 antibody CLP antibody CRTL1 antibody Crtl1l antibody HAPLN1 antibody HPLN1_HUMAN antibody Hyaluronan and proteoglycan link protein 1 antibody Hyaluronan and proteoglycan link protein 1 precursor antibody LP antibody Proteoglycan link protein antibody |
| Product description | The human cartilage link protein, also designated HAPLN1, is a member of the hyaluronan and proteoglycan link protein (HAPLN) family of proteins. This family contains 4 proteins with approximately 50% homology. The human cartilage link protein strengthens tissue architecture by using hyaluronic acid to stabilize the aggregates of proteoglycan monomers inside the extracellular cartilage matrix. It is widely expressed in most tissue types except brain tissue where, much like the other human cartilage link protein genes, it is weakly expressed. The HAPLN and the brain-specific CSPG genes, as well as other members of the link module superfamily, appear to have a common ancestral gene origin. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHC |
| WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRatZebra Fish |
| Concentration | 1mg/ml |
| Alternative Names | Cartilage link protein antibody cartilage linking protein 1 antibody Cartilage-link protein antibody Cartilage-linking protein 1 antibody CLP antibody CRTL1 antibody Crtl1l antibody HAPLN1 antibody HPLN1_HUMAN antibody Hyaluronan and proteoglycan link protein 1 antibody Hyaluronan and proteoglycan link protein 1 precursor antibody LP antibody Proteoglycan link protein antibody |
|---|---|
| Molecular Weight(MW) | 48 kDa |
| Cellular Localization | Secreted. |
Application
Fig1: Western blot analysis of HAPLN1 on different lysates using anti-HAPLN1 antibody at 1/1,000 dilution. Positive control: Lane 1: BT-20 Lane 2: HUVEC Lane 3: SW480
Application
Fig2: ICC staining HAPLN1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining HAPLN1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining HAPLN1 in BT-20 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HAPLN1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-HAPLN1 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-HAPLN1 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-HAPLN1 antibody. Counter stained with hematoxylin.| Positive Control | HUVEC, SW480, BT-20, human spleen tissue, human tonsil tissue, mouse spleen tissue, human liver tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
