Application
Fig1: Western blot analysis of FOXO1A on different lysates using anti-FOXO1A antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: NIH/3T3Application
Fig2: ICC staining FOXO1A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining FOXO1A in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining FOXO1A in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-FOXO1A antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-FOXO1A antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-FOXO1A antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-FOXO1A antibody. Counter stained with hematoxylin.| Product Name | FOXO1A [SU33-01] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FKH 1 antibody FKH1 antibody FKHR antibody Forkhead (Drosophila) homolog 1 (rhabdomyosarcoma) antibody Forkhead box O1 antibody Forkhead box protein O1 antibody Forkhead box protein O1A antibody Forkhead in rhabdomyosarcoma antibody Forkhead, Drosophila, homolog of, in rhabdomyosarcoma antibody FoxO transcription factor antibody foxo1 antibody FOXO1_HUMAN antibody FOXO1A antibody OTTHUMP00000018301 antibody |
| Product description | FKHR (for forkhead in rhabdomyosarcoma) and FKHRL1 are members of the forkhead family of transcription factors. Transcriptional activation of FKHR proteins is regulated by the serine/threonine kinase Akt1, which phosphorylates FKHRL1 and results in FKHRL1 associating with 14-3-3 proteins and being retained in the cytoplasm. Induction of apoptosis or withdrawal of growth factors stimulates dephosphorylation and nuclear translocation of FKHR proteins, leading to FKHR-induced gene-specific transcriptional activation. FKHR, also designated forkhead box protein O1A (FOXO1), is a ubiquitously expressed protein that shuttles between the cytoplasm and nucleus. Genetic mutations in FKHR genes, including the t(2;13) and t(1;3) translocations, are commonly found in alveolar rhabdomyosarcomas. These translocations result in the fusion of the amino terminus of Pax-3 or Pax-7, including the paired box and homeodomain DNA-binding domains, with the carboxy-terminus of FKHR, which contains a transcriptional activation domain. The Pax-3/FKHR fusion protein appears to function as an oncogenic transcription factor that enhances the activation of normal Pax-3 target genes. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIHC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRatZebra Fish |
| Concentration | 1mg/ml |
| Alternative Names | FKH 1 antibody
FKH1 antibody
FKHR antibody
Forkhead (Drosophila) homolog 1 (rhabdomyosarcoma) antibody
Forkhead box O1 antibody
Forkhead box protein O1 antibody
Forkhead box protein O1A antibody
Forkhead in rhabdomyosarcoma antibody
Forkhead Drosophila homolog of in rhabdomyosarcoma antibody FoxO transcription factor antibody foxo1 antibody FOXO1_HUMAN antibody FOXO1A antibody OTTHUMP00000018301 antibody |
|---|---|
| Molecular Weight(MW) | 80 kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
Application
Fig1: Western blot analysis of FOXO1A on different lysates using anti-FOXO1A antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: NIH/3T3
Application
Fig2: ICC staining FOXO1A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining FOXO1A in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining FOXO1A in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-FOXO1A antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-FOXO1A antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-FOXO1A antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-FOXO1A antibody. Counter stained with hematoxylin.| Positive Control | NIH/3T3, MCF-7, Hela, mouse brain tissue, human tonsil tissue, mouse heart tissue, human breast carcinoma tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
