Application
Fig 1: Western blot analysis of COX1/Cyclooxygenase 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.Positive control:Lane 1: 293T cell lysate, untreatedLane 2: NIH/3T3 cell lysate, untreated| Product Name | COX1/Cyclooxygenase 1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | COX 1 antibody COX 3 antibody COX-1 antibody COX1 antibody Cox3 antibody Cyclooxygenase 1 antibody Cyclooxygenase 3, included antibody Cyclooxygenase-1 antibody EC 1.14.99.1 antibody Partial COX1 proteins, included antibody PCOX1 antibody PGG/HS antibody PGH synthase 1 antibody PGH1_HUMAN antibody PGHS-1 antibody PGHS1 antibody PHS 1 antibody PHS1 antibody Prostaglandin G/H synthase 1 antibody Prostaglandin H2 synthase 1 antibody Prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase) antibody Prostaglandin-endoperoxide synthase 1 antibody PTGHS antibody PTGS1 antibody |
| Product description | Cytochrome c oxidase subunit I, COX1 (also designated COI, MTCO1 or oxidative phosphorylation (OxPhos) Complex IV, subunit I) is one of three mitochondrial DNA (mtDNA) encoded subunits (MTCO1-3) of respiratory Complex IV. Cytochrome c oxidase is a hetero-oligomeric enzyme composed of 13 subunits localized to the mitochondrial inner membrane and is the terminal enzyme complex of the electron transport chain. Complex IV catalyzes the reduction of molecular oxygen to water. The energy released is used to transport protons across the mitochondrial inner membrane. The resulting electro-chemical gradient is necessary for the synthesis of ATP. Complex IV contains 13 polypeptides; COX1, COX2 and COX3 (MTCO1-3) make up the catalytic core and are encoded by mtDNA while subunits IV, Va, Vb, VIa, VIb, VIc, VIIa, VIIb, VIIc and VIII are nuclear-encoded. |
| Immunogen | Synthetic peptide corresponding to C-terminal of Human COX1/Cyclooxygenase 1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500 |
|
| Species Reactivity | HumanMouse |
| Concentration | 2mg/ml |
| Alternative Names | COX 1 antibody
COX 3 antibody
COX-1 antibody
COX1 antibody
Cox3 antibody
Cyclooxygenase 1 antibody
Cyclooxygenase 3 included antibody Cyclooxygenase-1 antibody EC 1.14.99.1 antibody Partial COX1 proteins included antibody PCOX1 antibody PGG/HS antibody PGH synthase 1 antibody PGH1_HUMAN antibody PGHS-1 antibody PGHS1 antibody PHS 1 antibody PHS1 antibody Prostaglandin G/H synthase 1 antibody Prostaglandin H2 synthase 1 antibody Prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase) antibody Prostaglandin-endoperoxide synthase 1 antibody PTGHS antibody PTGS1 antibody |
|---|---|
| Molecular Weight(MW) | 69 kDa |
| Cellular Localization | Microsome membrane; ER membrane Peripheral membrane protein. |
Application
Fig 1: Western blot analysis of COX1/Cyclooxygenase 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.Positive control:Lane 1: 293T cell lysate, untreatedLane 2: NIH/3T3 cell lysate, untreated| Positive Control | NIH/3T3, 293T. |
|---|---|
| Application Notes | WB:1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 25%Glycerol. Preservative: 0.05% Sodium Azide. |
