Application
Fig1: Western blot analysis of Transmembrane protein 132A on mouse brain lysate lysate using anti-Transmembrane protein 132A antibody at 1/1,000 dilution.Application
Fig2: ICC staining Transmembrane protein 132A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Transmembrane protein 132A in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Transmembrane protein 132A antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of SH-SY-5Y cells with Transmembrane protein 132A antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | Transmembrane protein 132A |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GBP antibody HSPA5-binding protein 1 antibody HSPA5BP1 antibody KIAA1583 antibody T132A_HUMAN antibody Tmem132a antibody Transmembrane protein 132A antibody |
| Product description | TMEM132A is a member of TMEM132 family. Total of five members, namely TMEM132 A, B, C, D, E, were found. TMEM132A, also known as HSPA5-binding protein 1, may play a role in embryonic and postnatal development of the brain and regulate cAMP-induced GFAP gene expression via STAT3 phosphorylation. |
| Immunogen | Synthetic peptide within human Transmembrane protein 132A N-terminal. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | GBP antibody HSPA5-binding protein 1 antibody HSPA5BP1 antibody KIAA1583 antibody T132A_HUMAN antibody Tmem132a antibody Transmembrane protein 132A antibody |
|---|---|
| Molecular Weight(MW) | 55 kDa |
| Cellular Localization | Endoplasmic reticulum. Golgi apparatus. Membrane. |
Application
Fig1: Western blot analysis of Transmembrane protein 132A on mouse brain lysate lysate using anti-Transmembrane protein 132A antibody at 1/1,000 dilution.
Application
Fig2: ICC staining Transmembrane protein 132A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Transmembrane protein 132A in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Transmembrane protein 132A antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of SH-SY-5Y cells with Transmembrane protein 132A antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | Mouse brain tissue, Hela, SHG-44, SH-SY-5Y. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
