Application
Fig1: Western blot analysis of VANGL1 on MCF-7 cell lysate using anti-VANGL1 antibody at 1/500 dilution.Application
Fig2: ICC staining VANGL1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining VANGL1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining VANGL1in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Product Name | Vang-like protein 1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Vang-like protein 1 is a multi-pass membrane protein, expressed specifically in testis and ovary. It interacts through its C-terminal region with the N-terminal half of DVL1, DVL2 and DVL3. Vangl proteins play a key developmental role in establishing planar cell polarity (PCP) and in regulating convergent extension (CE) movements during embryogenesis. Vangl1 and Vangl2 are both downregulated in several cancer cell lines and primary tumors. Defects in VANGL1 are a cause of neural tube defects (NTD) and a cause of sacral defect with anterior meningocele (SDAM). |
| Immunogen | Synthetic peptide within human 100-160 aa. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB,ICC |
| WB:1:500 ICC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Alternative Names | ANGL planar cell polarity protein 1 antibody
KAI1 C-terminal interacting tetraspanin antibody
KITENIN antibody
Loop tail protein 2 homolog antibody
Loop-tail protein 2 homolog antibody
LPP2 antibody
MGC5338 antibody
STB2 antibody
STBM2 antibody
Strabismus 2 antibody
Strabismus Drosophila homolog of 2 antibody Van Gogh like protein 1 antibody Van Gogh Drosophila homolog of 1 antibody Van Gogh-like protein 1 antibody Vang like 1 (van gogh Drosophila) antibody Vang like protein 1 antibody Vang-like 1 antibody Vang-like protein 1 antibody VANG1_HUMAN antibody VANGL 1 antibody Vangl1 antibody |
|---|---|
| Molecular Weight(MW) | 80 kDa |
| Cellular Localization | Cell membrane. Membrane. |
| SwissProt ID | Q8TAA9 |
|---|
Application
Fig1: Western blot analysis of VANGL1 on MCF-7 cell lysate using anti-VANGL1 antibody at 1/500 dilution.
Application
Fig2: ICC staining VANGL1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining VANGL1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining VANGL1in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Positive Control | Hela, MCF-7, LOVO. |
|---|---|
| Application Notes | WB:1:500 ICC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 25%Glycerol. Preservative: 0.05% Sodium Azide. |
