Application
Fig1: Western blot analysis of CD80 on human brain tissue lysates using anti-CD80 antibody at 1/1,000 dilution.Application
Fig2: ICC staining CD80 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining CD80 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD80 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-CD80 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-CD80 antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of Hela cells with CD80 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | CD80/B7-1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Activation B7-1 antigen antibody B lymphocyte activation antigen B7 antibody B7 antibody B7-1 antibody B7-1 antigen antibody B7.1 antibody BB1 antibody CD28 antigen ligand 1 antibody CD28LG antibody CD28LG1 antibody CD80 antibody CD80 antigen (CD28 antigen ligand 1, B7-1 antigen) antibody CD80 antigen antibody CD80 molecule antibody CD80_HUMAN antibody Costimulatory factor CD80 antibody costimulatory molecule variant IgV-CD80 antibody CTLA-4 counter-receptor B7.1 antibody LAB7 antibody T-lymphocyte activation antigen CD80 antibody |
| Product description | B7-1 is a molecule found on activated B cells and monocytes which provides a costimulatory signal necessary for T cell activation and survival. After engagement of T-cell receptor with antigen in association with major histocompatibility complex class II, a second signal mediated through the binding of B7 to CD28 greatly upregulates the production of multiple lymphokines. B7-1 is a costimulatory molecule for the activation of both CD4+ and CD8+ T lymphocytes that prevents the induction of clonal anergy. Thus, the transfer of B7-1 genes into tumor cells can induce protective immunity and lead to tumor rejection of some tumors in model systems of in vivo tumor growth. |
| Immunogen | peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:500~1:2000 ICC:1:200~1:500 IHC:1:50~1:200 FC:1:200~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | Activation B7-1 antigen antibody
B lymphocyte activation antigen B7 antibody
B7 antibody
B7-1 antibody
B7-1 antigen antibody
B7.1 antibody
BB1 antibody
CD28 antigen ligand 1 antibody
CD28LG antibody
CD28LG1 antibody
CD80 antibody
CD80 antigen (CD28 antigen ligand 1 B7-1 antigen) antibody CD80 antigen antibody CD80 molecule antibody CD80_HUMAN antibody Costimulatory factor CD80 antibody costimulatory molecule variant IgV-CD80 antibody CTLA-4 counter-receptor B7.1 antibody LAB7 antibody T-lymphocyte activation antigen CD80 antibody |
|---|---|
| Molecular Weight(MW) | 60 kDa |
| Cellular Localization | Cell membrane |
| SwissProt ID | P33681 |
|---|
Application
Fig1: Western blot analysis of CD80 on human brain tissue lysates using anti-CD80 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining CD80 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining CD80 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD80 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-CD80 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-CD80 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of Hela cells with CD80 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | Human brain tissue lysates, N2A, SHG-44, human tonsil tissue, mouse lung tissue, mouse spleen tissue, Hela. |
|---|---|
| Application Notes | WB:1:500~1:2000 ICC:1:200~1:500 IHC:1:50~1:200 FC:1:200~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
