Application
Fig1: ELISA of three different PEGylated BSAs using anti-PEG RevMAb clone RM105, followed by an AP conjugated goat anti-rabbit IgG.Application
Fig2: Immunohistochemistry of mouse liver and kidney using 0.5 ug/mL of RevMAb anti-PEG Clone RM105. The mouse was injected with PEG-BSA or BSA for 3 hours before sampling.| Product Name | PEG (methoxy group) [1A4] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Polyethylene glycol (PEG) is a family of long chain polymers attached to a glycerine backbone. It is a nonionic, nontoxic, biocompatible, strongly hydrophilic polymer, which has a large exclusion volume in aqueous solution (1). The covalent attachment of PEG is now commonly used to modify a variety of proteins and drugs (2,3). The modification of a biopharmaceutical with PEG increases its hydrodynamic radius, reduces immunogenicity and proteolytic cleavage. Other benefits include decelerated renal excre-tion, improved stability towards proteolysis and increased solubility of the biopharmaceutical in aque-ous solutions (4). An Anti-PEG antibody can be used to monitor a drug’s pharmacokinetics, including distribution, metabolism and excretion. In addition, it can be used for the quality control of pegylated molecules in ELISA, WB and IHC. |
| Immunogen | KLH-PEG with terminal methoxy group |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHCICC/IF |
| WB:0.05ug/mL-1ug/mL IHC:0.5ug/mL-2ug/mL ELISA:0.01mg/mL – 0.3mg/mL |
|
| Species Reactivity | All |
| Concentration | 1mg/ml |
Application
Fig1: ELISA of three different PEGylated BSAs using anti-PEG RevMAb clone RM105, followed by an AP conjugated goat anti-rabbit IgG.
Application
Fig2: Immunohistochemistry of mouse liver and kidney using 0.5 ug/mL of RevMAb anti-PEG Clone RM105. The mouse was injected with PEG-BSA or BSA for 3 hours before sampling.| Positive Control | Mouse liver, Mouse kidney. |
|---|---|
| Application Notes | WB:0.05ug/mL-1ug/mL IHC:0.5ug/mL-2ug/mL ELISA:0.01mg/mL – 0.3mg/mL |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Stable for 1 Year from date of receipt. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
