Application
Fig1: Western blot analysis of Phospho-Histone H1.3(T17)+Histone H1.4(T17) on CRC cell lysates using anti-Phospho-Histone H1.3(T17)+Histone H1.4(T17) antibody at 1/500 dilution.Positive control: Lane 1: Untreated CRC whole cell lysatesLane2: CRC cells treated with 1.5ug/ml Colcemid for 12 hours whole cell lysatesApplication
Fig2: ICC staining Phospho-Histone H1.3(T17)+Histone H1.4(T17) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Phospho-Histone H1.3(T17)+Histone H1.4(T17) in CRC cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Phospho-Histone H1.3(T17)+Histone H1.4(T17) antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse colon cancer tissue using anti-Phospho-Histone H1.3(T17)+Histone H1.4(T17) antibody. Counter stained with hematoxylin.| Product Name | Phospho-Histone H1.3(T17)+Histone H1.4(T17) [SR38-03] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Histone H1.3 Histone H1c Histone H1s-2 HIST1H1DH1F3 Histone H1.4 Histone H1b Histone H1s-4 HIST1H1EH1F4 |
| Product description | Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation. |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Thr17 of human H1.4. |
| Modification | p-T17 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHC |
| WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Histone H1.3 Histone H1c Histone H1s-2 HIST1H1DH1F3 Histone H1.4 Histone H1b Histone H1s-4 HIST1H1EH1F4 |
|---|---|
| Molecular Weight(MW) | 30 kDa |
| Cellular Localization | Nucleus, Chromosome |
Application
Fig1: Western blot analysis of Phospho-Histone H1.3(T17)+Histone H1.4(T17) on CRC cell lysates using anti-Phospho-Histone H1.3(T17)+Histone H1.4(T17) antibody at 1/500 dilution.Positive control: Lane 1: Untreated CRC whole cell lysatesLane2: CRC cells treated with 1.5ug/ml Colcemid for 12 hours whole cell lysates
Application
Fig2: ICC staining Phospho-Histone H1.3(T17)+Histone H1.4(T17) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Phospho-Histone H1.3(T17)+Histone H1.4(T17) in CRC cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Phospho-Histone H1.3(T17)+Histone H1.4(T17) antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse colon cancer tissue using anti-Phospho-Histone H1.3(T17)+Histone H1.4(T17) antibody. Counter stained with hematoxylin.| Positive Control | NIH/3T3, CRC, human colon cancer tissue, mouse colon cancer tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
