Application
Fig1: Western blot analysis of Ferritin on zebrafish lysates using anti-Ferritin antibody at 1/1,000 dilution.Application
Fig2: ICC staining Ferritin in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Ferritin in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining Ferritin in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5:Western blot analysis of Ferritin on hybrid fish (crucian-carp) liver tissue lysate using anti-Ferritin antibody at 1/500 dilution.| Product Name | Ferritin [SC0620] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cell proliferation-inducing gene 15 protein antibody Ferritin H subunit antibody Ferritin heavy chain antibody Ferritin heavy polypeptide 1 antibody Ferritin L subunit antibody Ferritin light polypeptide antibody Ferritin, heavy polypeptide antibody FRIH_HUMAN antibody FTH antibody FTH1 antibody FTL antibody |
| Product description | Mammalian ferritins consist of 24 subunits made up of two types of polypeptide chains, ferritin heavy chain and ferritin light chain, which each have unique functions. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe (II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe (III). The most prominent role of mammalian ferritins is to provide iron-buffering capacity to cells. In addition to iron buffering, heavy chain ferritin is also involved in the regulation of thymidine biosynthesis via increased expression of cytoplasmic serine hydroxymethyltransferase, which is a limiting factor in thymidylate synthesis in MCF-7 cells. Light chain ferritin is involved in cataracts by at least two mechanisms, hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of ageing-related cataracts. The gene encoding human ferritin heavy chain maps to chromosome 11q13 and the human ferritin light chain gene maps to chromosome 19q13.3-q13.4. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IF |
| WB:1:500-1:1,000 ICC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRatZebra Fish |
| Concentration | 1mg/ml |
| Alternative Names | Cell proliferation-inducing gene 15 protein antibody
Ferritin H subunit antibody
Ferritin heavy chain antibody
Ferritin heavy polypeptide 1 antibody
Ferritin L subunit antibody
Ferritin light polypeptide antibody
Ferritin heavy polypeptide antibody FRIH_HUMAN antibody FTH antibody FTH1 antibody FTL antibody |
|---|---|
| Molecular Weight(MW) | 21 KDa |
| Cellular Localization | Cytoplasm. |
Application
Fig1: Western blot analysis of Ferritin on zebrafish lysates using anti-Ferritin antibody at 1/1,000 dilution.
Application
Fig2: ICC staining Ferritin in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Ferritin in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Ferritin in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5:Western blot analysis of Ferritin on hybrid fish (crucian-carp) liver tissue lysate using anti-Ferritin antibody at 1/500 dilution.| Positive Control | SHG-44, MCF-7, SH-SY-5Y, zebrafish. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
