Application
Fig1: Western blot analysis of ADIPOR1 on human skeletal muscle lysates using anti-ADIPOR1 antibody at 1/1,000 dilution.Application
Fig2: ICC staining ADIPOR1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining ADIPOR1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining ADIPOR1 in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ADIPOR1 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-ADIPOR1 antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of HepG2 cells with ADIPOR1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | Adiponectin receptor protein 1 [SC69-04] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Adiponectin is a circulating hormone secreted by adipocytes that improves the metabolism of glucose and lipids, and is expressed at low levels in those with obesity and diabetes. Adiponectin receptors AdipoR1 and AdipoR2, also designated progestin and adipoQ receptor family members I and II, respectively, regulate fatty acid oxidation and the uptake of glucose by adiponectin. Each receptor activates a unique set of signaling molecules including AMPK, p38 MAPK and PPARα. AdipoR1 has a high-affinity for globular adiponectin and low-affinity for full-length adiponectin, while AdipoR2 has an intermediate affinity for both forms. AdipoR1 and AdipoR2 are mainly expressed in liver and muscle. Adiponectin, AdipoR1 and AdipoR2 are all associated with body composition, Insulin sensitivity, and metabolic parameters. Physical training increases circulating adiponectin and mRNA expression of AdipoR1 and AdipoR2 in muscle, which may mediate the improvement of Insulin resistance and the metabolic syndrome in response to exercise. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB,ICC/IF,IHC,FC |
| WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human,Mouse,Rat,Zebra Fish |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Alternative Names | ACDCR1 antibody ADIPO R1 antibody Adiponectin receptor protein 1 antibody ADIPOR 1 antibody Adipor1 antibody ADR1_HUMAN antibody CGI 45 antibody CGI 45 protein antibody CGI-45 antibody CGI45 antibody CGI45 protein antibody FLJ25385 antibody FLJ42464 antibody PAQR1 antibody Progestin and adipoQ receptor family member I antibody TESBP1A antibody |
|---|---|
| Molecular Weight(MW) | 43 kDa |
| Cellular Localization | Cell membrane. |
Application
Fig1: Western blot analysis of ADIPOR1 on human skeletal muscle lysates using anti-ADIPOR1 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining ADIPOR1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining ADIPOR1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining ADIPOR1 in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ADIPOR1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-ADIPOR1 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of HepG2 cells with ADIPOR1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | LO2, Hela, HepG2, human kidney tissue, mouse liver tissue, human skeletal muscle tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
