Application
Fig1: Western blot analysis of Rab4A on different cell lysates using anti-Rab4A antibody at 1/500 dilution. Positive control: Lane 1: MCF-7 Lane 2: 293TApplication
Fig2: ICC staining Rab4A in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Product Name | Rab4A [SR27-08] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HRES 1 / RAB4 antibody Oncogene RAB4 antibody Rab 4 antibody RAB 4A antibody RAB4 member RAS oncogene family antibody Rab4a antibody RAB4A member RAS oncogene family antibody RAB4A_HUMAN antibody Ras related protein Rab 4A antibody Ras related protein Rab4A antibody Ras-related protein Rab-4A antibody |
| Product description | The Ras-related superfamily of guanine nucleotide binding proteins, which includes the R-Ras, Rap, Ral/Rec and Rho/Rab superfamilies, exhibits 30-60% homology with Ras p21. Accumulating data suggests an important role for Rab proteins, either in endocytosis or in biosynthetic protein transport. The transport of newly synthesized proteins from the endoplasmic reticulum to various stacks of the Golgi complex and to secretory vesicles involves at each stage the movement of carrier vesicles, a process that appears to involve Rab protein function. The possiblity that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40% homologous to Rab proteins, is associated with secretory vesicles. At least eight members of the Rab family have been identified, each of which is found at a particular stage of a membrane transport pathway. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIP |
| WB:1:500-1:1,000 ICC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | HRES 1 / RAB4 antibody Oncogene RAB4 antibody Rab 4 antibody RAB 4A antibody RAB4 member RAS oncogene family antibody Rab4a antibody RAB4A member RAS oncogene family antibody RAB4A_HUMAN antibody Ras related protein Rab 4A antibody Ras related protein Rab4A antibody Ras-related protein Rab-4A antibody |
|---|---|
| Molecular Weight(MW) | 24 kDa |
| Cellular Localization | Cytoplasm, Membrane |
Application
Fig1: Western blot analysis of Rab4A on different cell lysates using anti-Rab4A antibody at 1/500 dilution. Positive control: Lane 1: MCF-7 Lane 2: 293T
Application
Fig2: ICC staining Rab4A in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Positive Control | PC-12, MCF-7. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
