Application
Fig1: Western blot analysis of NSE on different lysates using anti-NSE antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2 Lane 2: Hela Lane 3: 293Application
Fig2: ICC staining NSE in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining NSE in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-NSE antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-NSE antibody. Counter stained with hematoxylin.Application
Fig6: Western blot analysis of NSE on hybrid fish (crucian-carp) brain tissue lysates using anti-NSE antibody at 1/500 dilution.| Product Name | NSE [SC06-28] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | d as highly conserved cytoplasmic glycolytic enzymes that may be involved in differentiation. Three isoenzymes have been identified, α Enolase, β Enolase and γ Enolase. α Enolase expression has been detected on most tissues, whereas β Enolase is expressed predominantly in muscle tissue and γ Enolase is detected only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB,ICC,IHC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | Human,Mouse,Rat,Zebra Fish |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Alternative Names | 2 phospho D glycerate hydrolyase antibody
2-phospho-D-glycerate hydro-lyase antibody Eno 2 antibody ENO2 antibody ENOG antibody ENOG_HUMAN antibody Enolase 2 (gamma neuronal) antibody Enolase 2 antibody Enolase 2 gamma neuronal antibody Enolase2 antibody Epididymis secretory protein Li 279 antibody Gamma enolase antibody Gamma-enolase antibody HEL S 279 antibody Neural enolase antibody Neuron specific enolase antibody Neuron specific gamma enolase antibody Neuron-specific enolase antibody Neurone specific enolase antibody NSE antibody |
|---|---|
| Molecular Weight(MW) | 47 kDa |
| Cellular Localization | Cytoplasm, Cell membrane. |
Application
Fig1: Western blot analysis of NSE on different lysates using anti-NSE antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2 Lane 2: Hela Lane 3: 293
Application
Fig2: ICC staining NSE in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining NSE in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-NSE antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-NSE antibody. Counter stained with hematoxylin.
Application
Fig6: Western blot analysis of NSE on hybrid fish (crucian-carp) brain tissue lysates using anti-NSE antibody at 1/500 dilution.| Positive Control | SH-SY-5Y, 293, Hela, HepG2, rat brain tissue, mouse brain tissue, human tonsil tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
