Application
Fig1: Western blot analysis of PUMA on different lysates using anti-PUMA antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: K562Application
Fig2: ICC staining PUMA in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining PUMA in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PUMA antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human gastric carcimnoma tissue using anti-PUMA antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-PUMA antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-PUMA antibody. Counter stained with hematoxylin.Application
Fig8: Flow cytometric analysis of Hela cells with PUMA antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | PUMA [SR42-09] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The expression of PUMA is regulated by the tumor suppressor p53. PUMA is involved in p53-dependent and -independent apoptosis induced by a variety of signals, and is regulated by transcription factors, not by post-translational modifications. After activation, PUMA interacts with antiapoptotic Bcl-2 family members, thus freeing Bax and/or Bak which are then able to signal apoptosis to the mitochondria. Following mitochondrial dysfunction, the caspase cascade is activated ultimately leading to cell death. Several studies have shown that PUMA function is affected or absent in cancer cells. Additionally, many human tumors contain p53 mutations, which results in no induction of PUMA, even after DNA damage induced through irradiation or chemotherapy drugs.Other cancers, which exhibit overexpression of antiapotptic Bcl-2 family proteins, counteract and overpower PUMA-induced apoptosis. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB,ICC/IF,IHC,FC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Alternative Names | BBC 3 antibody Bbc3 antibody BBC3_HUMAN antibody BCL 2 binding component 3 antibody Bcl-2-binding component 3 antibody BCL2 binding component 3 antibody JFY 1 antibody JFY-1 antibody JFY1 antibody p53 up regulated modulator of apoptosis antibody p53 up-regulated modulator of apoptosis antibody p53 Upregulated Modulator of Apoptosis antibody PUMA alpha antibody PUMA/JFY1 antibody |
|---|---|
| Molecular Weight(MW) | 18 kDa |
| Cellular Localization | Mitochondrion. |
Application
Fig1: Western blot analysis of PUMA on different lysates using anti-PUMA antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: K562
Application
Fig2: ICC staining PUMA in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining PUMA in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PUMA antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human gastric carcimnoma tissue using anti-PUMA antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-PUMA antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-PUMA antibody. Counter stained with hematoxylin.
Application
Fig8: Flow cytometric analysis of Hela cells with PUMA antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | K562, SKOV-3, Hela, human breast carcinoma tissue, mouse small intestine tissue, human gastric carcimnoma tissue, mouse stomach tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
