Application
Fig1: Western blot analysis of PI 3 Kinase p55 γ on different lysates using anti-PI 3 Kinase p55 γ antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7 Lane 2: Jurkat Lane 3: Mouse testisApplication
Fig2: ICC staining PI 3 Kinase p55 γ in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining PI 3 Kinase p55 γ in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PI 3 Kinase p55 γ antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PI 3 Kinase p55 γ antibody. Counter stained with hematoxylin.| Product Name | PI 3 Kinase p55 gamma [SR44-06] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DKFZp686P05226 antibody FLJ41892 antibody OTTHUMP00000009783 antibody OTTHUMP00000009786 antibody p55 antibody p55 gamma antibody P55G_HUMAN antibody p55PIK antibody Phosphatidylinositol 3 kinase regulatory subunit gamma antibody Phosphatidylinositol 3 kinase regulatory subunit polypeptide 3 antibody Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 3 (p55, gamma) antibody Phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma antibody Phosphatidylinositol 3-kinase regulatory subunit gamma antibody Phosphoinositide 3 kinase regulatory subunit 3 (gamma) antibody Phosphoinositide 3 kinase regulatory subunit 3 antibody Phosphoinositide 3 kinase regulatory subunit polypeptide 3 antibody Phosphoinositide 3 kinase, regulatory subunit 3 (p55, gamma) antibody Phosphoinositide 3 kinase, regulatory subunit, polypeptide 3 (p55, gamma) antibody PI3 kinase p85 subunit gamma antibody PI3-kinase regulatory subunit gamma antibody PI3-kinase subunit p55-gamma antibody PI3K regulatory subunit gamma antibody Pik3r3 antibody PtdIns 3 kinase p85 gamma antibody PtdIns-3-kinase regulatory subunit gamma antibody PtdIns-3-kinase regulatory subunit p55-gamma antibody |
| Product description | Phosphatidylinositol 3-kinase is a lipid kinase that phosphorylates the inositol ring of phosphatidylinositol and related compounds at the 3’ position. PI 3-kinase p55γ (PIK3R3) is comprised of a catalytic subunit and a regulatory subunit. The human p55γ protein is composed of a rare amino terminal region followed by a proline-rich motif and two Src homology 2 (SH2) domains. PI 3-kinase p55γ mRNAs are expressed in most human fetal and adult tissues; predominant expression is observed in the adult testis. Splice variant(s) of PI 3-kinase p55γ have been identified; one of which has a deletion of 36 amino acids at the amino terminus and another which has an insertion of 59 amino acids at position 256 between the SH2 domains. Research suggests that PI 3-kinase p55γ interacts with the IGFIR (Insulin-like growth factor-I receptor) and IR (Insulin receptor) and may be involved in PI 3-kinase activation by these receptors. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHC |
| WB:1:1,000-1:10,000 ICC:1:50-1:200 IHC:1:200-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | DKFZp686P05226 antibody
FLJ41892 antibody
OTTHUMP00000009783 antibody
OTTHUMP00000009786 antibody
p55 antibody
p55 gamma antibody
P55G_HUMAN antibody
p55PIK antibody
Phosphatidylinositol 3 kinase regulatory subunit gamma antibody
Phosphatidylinositol 3 kinase regulatory subunit polypeptide 3 antibody
Phosphatidylinositol 3 kinase regulatory subunit polypeptide 3 (p55 gamma) antibody Phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma antibody Phosphatidylinositol 3-kinase regulatory subunit gamma antibody Phosphoinositide 3 kinase regulatory subunit 3 (gamma) antibody Phosphoinositide 3 kinase regulatory subunit 3 antibody Phosphoinositide 3 kinase regulatory subunit polypeptide 3 antibody Phosphoinositide 3 kinase regulatory subunit 3 (p55 gamma) antibody Phosphoinositide 3 kinase regulatory subunit polypeptide 3 (p55 gamma) antibody PI3 kinase p85 subunit gamma antibody PI3-kinase regulatory subunit gamma antibody PI3-kinase subunit p55-gamma antibody PI3K regulatory subunit gamma antibody Pik3r3 antibody PtdIns 3 kinase p85 gamma antibody PtdIns-3-kinase regulatory subunit gamma antibody PtdIns-3-kinase regulatory subunit p55-gamma antibody |
|---|---|
| Molecular Weight(MW) | 54 kDa |
| Cellular Localization | Cytosol. |
Application
Fig1: Western blot analysis of PI 3 Kinase p55 γ on different lysates using anti-PI 3 Kinase p55 γ antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7 Lane 2: Jurkat Lane 3: Mouse testis
Application
Fig2: ICC staining PI 3 Kinase p55 γ in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining PI 3 Kinase p55 γ in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PI 3 Kinase p55 γ antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PI 3 Kinase p55 γ antibody. Counter stained with hematoxylin.| Positive Control | PC-3M, MCF-7, SH-SY-5Y, Jurkat, mouse testis tissue, mouse brain tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:10,000 ICC:1:50-1:200 IHC:1:200-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
