Application
Fig1: Western blot analysis of Prohibitin on different lysates using anti-Prohibitin antibody at 1/1,000 dilution. Positive control: Lane 1: 293 Lane 2: Jurkat Lane 3: Mouse kidney Lane 4: HepG2Application
Fig2: ICC staining Prohibitin in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Prohibitin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Prohibitin antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Prohibitin antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Prohibitin antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Prohibitin antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-Prohibitin antibody. Counter stained with hematoxylin.Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Prohibitin antibody. Counter stained with hematoxylin.Application
Fig10: Flow cytometric analysis of HepG2 cells with Prohibitin antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the seco antibodyApplication
Fig11:Western blot analysis of Prohibitin on hybrid fish (crucian-carp) liver tissue lysate using anti-Prohibitin antibody at 1/500 dilution.| Product Name | Prohibitin [SR46-02] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Epididymis luminal protein 215 antibody Epididymis secretory sperm binding protein Li 54e antibody HEL 215 antibody HEL S 54e antibody PHB antibody PHB_HUMAN antibody PHB1 antibody Prohibitin antibody |
| Product description | Prohibitin is an evolutionarily conserved protein that has antiproliferative activity. The gene encoding human prohibitin maps to chromosome 17q21 and is ubiquitously expressed. Prohibitin is a post-synthetically modified protein that is localized in the inner membrane of mitochondria, where it regulates the cell cycle by blocking the transition between the G1 and S phases, and on the plasma membrane of B cells, where it mediates B cell maturation. Prohibitin mRNA and protein levels are high in G1, decline during the S phase, rise again in G2 and decline in M phase, which suggests that prohibitin controls the cell cycle by using both transcriptional and posttranslational mechanisms. Prohibitin is also a potential tumor suppressor protein that binds to retinoblastoma (Rb) and subsequently inhibits the activity of E2F family members in response to specific signaling cascades. Prohibitin 2 is a repressor of estrogen receptor activity, and is required for somatic and germline differentiation in the larval gonad during embryonic development. Mutations in the Prohibitin genes are correlated with breast cancer development and/or progressionin more than 80% of the cell lines analyzed. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIHCIPFC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:200-1:500 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRatZebra Fish |
| Concentration | 1mg/ml |
| Alternative Names | Epididymis luminal protein 215 antibody Epididymis secretory sperm binding protein Li 54e antibody HEL 215 antibody HEL S 54e antibody PHB antibody PHB_HUMAN antibody PHB1 antibody Prohibitin antibody |
|---|---|
| Molecular Weight(MW) | 30 kDa |
| Cellular Localization | Mitochondrion inner membrane. |
Application
Fig1: Western blot analysis of Prohibitin on different lysates using anti-Prohibitin antibody at 1/1,000 dilution. Positive control: Lane 1: 293 Lane 2: Jurkat Lane 3: Mouse kidney Lane 4: HepG2
Application
Fig2: ICC staining Prohibitin in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Prohibitin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Prohibitin antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Prohibitin antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Prohibitin antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Prohibitin antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-Prohibitin antibody. Counter stained with hematoxylin.
Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Prohibitin antibody. Counter stained with hematoxylin.
Application
Fig10: Flow cytometric analysis of HepG2 cells with Prohibitin antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the seco antibody
Application
Fig11:Western blot analysis of Prohibitin on hybrid fish (crucian-carp) liver tissue lysate using anti-Prohibitin antibody at 1/500 dilution.| Positive Control | NIH/3T3, Jurkat, HepG2, human liver cancer tissue, mouse stomach tissue, human kidney tissue, mouse heart tissue, mouse liver tissue, mouse kidney tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:200-1:500 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
